Human Factor IX Transgenic Sheep Produced by Transfer of Nuclei from Transfected Fetal Fibroblasts

Angelika E. Schnieke; Alexander J. Kind; William A. Ritchie; Karen Mycock; Angela R. Scott; Marjorie Ritchie; Ian Wilmut; Alan Colman; Keith H. S. Campbell

doi:10.1126/science.278.5346.2130

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Human Factor IX Transgenic Sheep Produced by Transfer of Nuclei from Transfected Fetal Fibroblasts

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Human Factor IX Transgenic Sheep Produced by Transfer of Nuclei from Transfected Fetal Fibroblasts

Angelika E. Schnieke, Alexander J. Kind, William A. Ritchie, Karen Mycock, Angela R. Scott, Marjorie Ritchie, Ian Wilmut, Alan Colman et Keith H. S. Campbell

Science (American Association for the Advancement of Science), Vol.278(5346), pp.2130-2133

19/12/1997

DOI: https://doi.org/10.1126/science.278.5346.2130

PMID: 9405350

Résumé

Ovine primary fetal fibroblasts were cotransfected with a neomycin resistance marker gene ( neo ) and a human coagulation factor IX genomic construct designed for expression of the encoded protein in sheep milk. Two cloned transfectants and a population of neomycin (G418)–resistant cells were used as donors for nuclear transfer to enucleated oocytes. Six transgenic lambs were liveborn: Three produced from cloned cells contained factor IX and neo transgenes, whereas three produced from the uncloned population contained the marker gene only. Somatic cells can therefore be subjected to genetic manipulation in vitro and produce viable animals by nuclear transfer. Production of transgenic sheep by nuclear transfer requires fewer than half the animals needed for pronuclear microinjection.

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Titre: Human Factor IX Transgenic Sheep Produced by Transfer of Nuclei from Transfected Fetal Fibroblasts
Créateurs - sans rôle: Angelika E. Schnieke - A. E. Schnieke, A. J. Kind, K. Mycock, A. R. Scott, A. Colman, PPL Therapeutics, Roslin, Midlothian, EH25 9PP, Scotland, UK., W. A. Ritchie, M. Ritchie, I. Wilmut, K. H. S. Campbell, Roslin Institute, Roslin, Midlothian, EH25 9PS, Scotland, UK
Alexander J. Kind - A. E. Schnieke, A. J. Kind, K. Mycock, A. R. Scott, A. Colman, PPL Therapeutics, Roslin, Midlothian, EH25 9PP, Scotland, UK., W. A. Ritchie, M. Ritchie, I. Wilmut, K. H. S. Campbell, Roslin Institute, Roslin, Midlothian, EH25 9PS, Scotland, UK
William A. Ritchie - A. E. Schnieke, A. J. Kind, K. Mycock, A. R. Scott, A. Colman, PPL Therapeutics, Roslin, Midlothian, EH25 9PP, Scotland, UK., W. A. Ritchie, M. Ritchie, I. Wilmut, K. H. S. Campbell, Roslin Institute, Roslin, Midlothian, EH25 9PS, Scotland, UK
Karen Mycock - A. E. Schnieke, A. J. Kind, K. Mycock, A. R. Scott, A. Colman, PPL Therapeutics, Roslin, Midlothian, EH25 9PP, Scotland, UK., W. A. Ritchie, M. Ritchie, I. Wilmut, K. H. S. Campbell, Roslin Institute, Roslin, Midlothian, EH25 9PS, Scotland, UK
Angela R. Scott - A. E. Schnieke, A. J. Kind, K. Mycock, A. R. Scott, A. Colman, PPL Therapeutics, Roslin, Midlothian, EH25 9PP, Scotland, UK., W. A. Ritchie, M. Ritchie, I. Wilmut, K. H. S. Campbell, Roslin Institute, Roslin, Midlothian, EH25 9PS, Scotland, UK
Marjorie Ritchie - A. E. Schnieke, A. J. Kind, K. Mycock, A. R. Scott, A. Colman, PPL Therapeutics, Roslin, Midlothian, EH25 9PP, Scotland, UK., W. A. Ritchie, M. Ritchie, I. Wilmut, K. H. S. Campbell, Roslin Institute, Roslin, Midlothian, EH25 9PS, Scotland, UK
Ian Wilmut - A. E. Schnieke, A. J. Kind, K. Mycock, A. R. Scott, A. Colman, PPL Therapeutics, Roslin, Midlothian, EH25 9PP, Scotland, UK., W. A. Ritchie, M. Ritchie, I. Wilmut, K. H. S. Campbell, Roslin Institute, Roslin, Midlothian, EH25 9PS, Scotland, UK
Alan Colman - A. E. Schnieke, A. J. Kind, K. Mycock, A. R. Scott, A. Colman, PPL Therapeutics, Roslin, Midlothian, EH25 9PP, Scotland, UK., W. A. Ritchie, M. Ritchie, I. Wilmut, K. H. S. Campbell, Roslin Institute, Roslin, Midlothian, EH25 9PS, Scotland, UK
Keith H. S. Campbell - A. E. Schnieke, A. J. Kind, K. Mycock, A. R. Scott, A. Colman, PPL Therapeutics, Roslin, Midlothian, EH25 9PP, Scotland, UK., W. A. Ritchie, M. Ritchie, I. Wilmut, K. H. S. Campbell, Roslin Institute, Roslin, Midlothian, EH25 9PS, Scotland, UK
Détails de publication: Science (American Association for the Advancement of Science), Vol.278(5346), pp.2130-2133
Nombre de pages: 4
Identifiants: 99139668409311
Unité académique: Institut de Génétique Humaine - IGH
Langue: English
Type de ressource: Journal article