Abstract
Reference values for the non-specific immune response of stickleback have been developed to better understand the natural variability of the immunomarkers and to increase their relevance for the detection of environmental perturbations. However, under field conditions, temperature and salinity can vary from station to station and their influence on the reference ranges of the immunomarkers should therefore be quantified. To this end, adult sticklebacks were exposed either to different temperatures (from 12 to 18 °C) or to different salinities (from 0 to 30 g/L) for 21 days after 10 days of acclimatization. The results were then projected onto reference ranges to better determine the effect of temperature and salinity on the innate immune response. With the exception of leucocyte necrosis at higher temperature and respiratory burst at lower temperature, previously established reference ranges for immunomarkers of sticklebacks were suitable when variations in temperature and salinity were tested. Finally, this study argues for the possibility of using stickleback and its immune reference range in the field regardless of temperature and salinity, due to its relatively temperature and salinity independent innate immune response. Reference ranges for immunomarkers in stickleback could be a real added value to water quality diagnosis in biomonitoring programs in variable seasonal and geographical environmental contexts. Furthermore, these results confirm the rapid adaptability of sticklebacks to different variations in temperature and salinity without affecting their immunological parameters.