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High-resolution mass spectrometry of small molecules bound to membrane proteins
Article de revue   Avec comité de lecture

High-resolution mass spectrometry of small molecules bound to membrane proteins

Joseph Gault, Joseph A. C. Donlan, Idlir Liko, Jonathan T. S. Hopper, Kallol Gupta, Nicholas G. Housden, Weston B. Struwe, Michael T. Marty, Todd Mize, Cherine Bechara, …
Nature methods, Vol.13(4), pp.333-336
01/04/2016
DOI: https://doi.org/10.1038/NMETH.3771
PMID: 26901650

Résumé

Biochemical Research Methods Biochemistry & Molecular Biology Life Sciences & Biomedicine Science & Technology
Small molecules are known to stabilize membrane proteins and to modulate their function and oligomeric state, but such interactions are often hard to precisely define. Here we develop and apply a high-resolution, Orbitrap mass spectrometry-based method for analyzing intact membrane protein-ligand complexes. Using this platform, we resolve the complexity of multiple binding events, quantify small molecule binding and reveal selectivity for endogenous lipids that differ only in acyl chain length.

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