Résumé
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A novel phosphoramidite methodology allowing the formation of
H-phosphonate oligonucleotides was employed to introduce backbone modifications into oligonucleotides. Novel
N,
N-diisopropylamino-
para-methoxybenzylphosphoramidites were prepared and used in a two-step synthesis cycle during the elongation process. The coupling step was directly followed by an acidic treatment performing concomitant deprotection and phosphite deprotection through an Arbuzov reaction to yield an
H-phosphonate linkage.