Résumé
AIM:To investigate whether Src,JAK2 and phosphatidylinositol 3-kinase (PI3K) pathways are involved in the proliferation of human colonic tumour cells induced by glycine-extended gastrin (G-gly),the precursor of the mature amidated gastrin and to elucidate the molecular interaction between these three kinases in response to this peptide.METHODS:Using the human colonic tumour cell line HCT116 as a model,we first measured the activation of PI3K,p60-Src and JAK2 in response to G-gly by in vitro kinase assays.Then we investigated the involvement of these kinases in G-gly-induced cell proliferation by MTT test.RESULTS:G-gly stimulation induced p60-Src,JAK2 and PI3K activation in HCT116.The different pathways were involved in proliferation of human colon cancer cells induced by G-gly.Furthermore,we found that both Src and JAK2 were necessary to PI3K regulation by this peptide.However,we did not find any cross-talk between the two tyrosine kinases.CONCLUSION:Our results suggest that the p60-Src/PI3K and JAK2/PI3K pat