Résumé
In order to define transmission routes of cryptosporidiosis and develop markers that distinguish
Cryptosporidium parvum isolates, we have identified 2 polymorphic restriction enzyme sites in a
C. parvum repetitive DNA sequence. The target sequence was amplified by polymerase chain reaction from 100 to 500 oocysts and the amplified product was subjected to restriction enzyme digestion. Typing of 23 isolates showed that
10
10
calf isolates had the same profile. In contrast, 2 patterns were observed among human isolates:
7
13
displayed the calf profile, and
6
13
presented another pattern. The PCR-RFLP assay described here is a sensitive tool to distinguish
C. parvum isolates.