Genomic Analysis of the Necrotrophic Fungal Pathogens Sclerotinia sclerotiorum and Botrytis cinerea

Joelle Amselem; Christina A. Cuomo; Jan A. L. van Kan; Muriel Viaud; Ernesto P. Benito; Arnaud Couloux; Pedro Coutinho; Ronald P. de Vries; Paul S. Dyer; Sabine, Helma Fillinger-David; Elisabeth Fournier; Lilian Gout; Matthias Hahn; Linda Kohn; Nicolas Lapalu; Kim M. Plummer; Jean-Marc Pradier; Emmanuel Quevillon; Amir Sharon; Adeline Simon; Arjen ten Have; Bettina Tudzynski; Paul Tudzynski; Patrick Wincker; Marion Andrew; Véronique Anthouard; Ross E. Beever; Rolland Beffa; Isabelle Benoit; Ourdia Bouzid; Baptiste Brault; Zehua Chen; Mathias Choquer; Jerome Collemare; Pascale Cotton; Etienne Danchin; Corinne da Silva; Angelique Gautier; Corinne Giraud-Delville; Tatiana Giraud; Celedonio Gonzalez; Sandrine Grossetete; Ulrich Gueldener; Bernard Henrissat; Barbara J. Howlett; Chinnappa Kodira; Matthias Kretschmer; Anne Lappartient; Michaela Leroch; Caroline Levis; Evan Mauceli; Cécile Neuvéglise; Birgitt Oeser; Matthew Pearson; Julie Poulain; Nathalie Poussereau; Hadi Quesneville; Christine Rascle; Julia Schumacher; Béatrice Segurens; Adrienne Sexton; Evelyn Silva; Catherine Sirven; Darren M. Soanes; Nicholas J. Talbot; Matt Templeton; Chandri Yandava; Oded Yarden; Qiandong Zeng; Jeffrey A. Rollins; Marc-Henri Lebrun; Marty Dickman

doi:10.1371/journal.pgen.1002230

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Genomic Analysis of the Necrotrophic Fungal Pathogens Sclerotinia sclerotiorum and Botrytis cinerea

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Genomic Analysis of the Necrotrophic Fungal Pathogens Sclerotinia sclerotiorum and Botrytis cinerea

Joelle Amselem, Christina A. Cuomo, Jan A. L. van Kan, Muriel Viaud, Ernesto P. Benito, Arnaud Couloux, Pedro Coutinho, Ronald P. de Vries, Paul S. Dyer, Sabine, Helma Fillinger-David, …

PLoS genetics, Vol.7(8), p.e1002230

01/08/2011

DOI: https://doi.org/10.1371/journal.pgen.1002230

PMID: 21876677

Résumé

Life Sciences

Sclerotinia sclerotiorum and Botrytis cinerea are closely related necrotrophic plant pathogenic fungi notable for their wide host ranges and environmental persistence. These attributes have made these species models for understanding the complexity of necrotrophic, broad host-range pathogenicity. Despite their similarities, the two species differ in mating behaviour and the ability to produce asexual spores. We have sequenced the genomes of one strain of S. sclerotiorum and two strains of B. cinerea. The comparative analysis of these genomes relative to one another and to other sequenced fungal genomes is provided here. Their 38-39 Mb genomes include 11,860-14,270 predicted genes, which share 83% amino acid identity on average between the two species. We have mapped the S. sclerotiorum assembly to 16 chromosomes and found large-scale co-linearity with the B. cinerea genomes. Seven percent of the S. sclerotiorum genome comprises transposable elements compared to <1% of B. cinerea. The arsenal of genes associated with necrotrophic processes is similar between the species, including genes involved in plant cell wall degradation and oxalic acid production. Analysis of secondary metabolism gene clusters revealed an expansion in number and diversity of B. cinerea-specific secondary metabolites relative to S. sclerotiorum. The potential diversity in secondary metabolism might be involved in adaptation to specific ecological niches. Comparative genome analysis revealed the basis of differing sexual mating compatibility systems between S. sclerotiorum and B. cinerea. The organization of the mating-type loci differs, and their structures provide evidence for the evolution of heterothallism from homothallism. These data shed light on the evolutionary and mechanistic bases of the genetically complex traits of necrotrophic pathogenicity and sexual mating. This resource should facilitate the functional studies designed to better understand what makes these fungi such successful and persistent pathogens of agronomic crops.

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Titre: Genomic Analysis of the Necrotrophic Fungal Pathogens Sclerotinia sclerotiorum and Botrytis cinerea
Créateurs - sans rôle: Joelle Amselem - Unité de Recherche Génomique Info
Christina A. Cuomo - Broad Institute
Jan A. L. van Kan - Wageningen University and Research [Wageningen]
Muriel Viaud - BIOlogie et GEstion des Risques en agriculture
Ernesto P. Benito - Departamento de Microbiología y Genética, Centro Hispano-Luso de Investigaciones Agrarias
Arnaud Couloux - Genoscope - Centre national de séquençage [Evry]
Pedro Coutinho - Architecture et Fonction des Macromolécules Biologiques
Ronald P. de Vries - Kluyver Centre for Genomics of Industrial Fermentations
Paul S. Dyer - School of Biology
Sabine, Helma Fillinger-David - BIOlogie et GEstion des Risques en agriculture
Elisabeth Fournier - BIOlogie et GEstion des Risques en agriculture
Lilian Gout - BIOlogie et GEstion des Risques en agriculture
Matthias Hahn - Faculty of Biology
Linda Kohn - Biology Department
Nicolas Lapalu - Unité de Recherche Génomique Info
Kim M. Plummer - Botany Department
Jean-Marc Pradier - BIOlogie et GEstion des Risques en agriculture
Emmanuel Quevillon - Unité de Recherche Génomique Info
Amir Sharon - Department of Molecular Microbiology and Biotechnology
Adeline Simon - BIOlogie et GEstion des Risques en agriculture
Arjen ten Have - Instituto de Investigaciones Biológicas [Mar del Plata]
Bettina Tudzynski - Molekularbiologie und Biotechnologie der Pilze
Paul Tudzynski - Molekularbiologie und Biotechnologie der Pilze
Patrick Wincker - Genoscope - Centre national de séquençage [Evry]
Marion Andrew - Biology Department
Véronique Anthouard - Genoscope - Centre national de séquençage [Evry]
Ross E. Beever - Manaaki Whenua – Landcare Research [Lincoln]
Rolland Beffa - Microbiologie, adaptation et pathogénie
Isabelle Benoit - Kluyver Centre for Genomics of Industrial Fermentations
Ourdia Bouzid - Kluyver Centre for Genomics of Industrial Fermentations
Baptiste Brault - Biologie et Gestion des Risques en Agriculture
Zehua Chen - Broad Institute
Mathias Choquer - BIOlogie et GEstion des Risques en agriculture
Jerome Collemare - Laboratory of Phytopathology
Pascale Cotton - Laboratoire de Génomique Fonctionnelle des Champignons Pathogènes de Plantes
Etienne Danchin - Interactions Biotiques et Santé Végétale
Corinne da Silva - Genoscope - Centre national de séquençage [Evry]
Angelique Gautier - BIOlogie et GEstion des Risques en agriculture
Corinne Giraud-Delville - BIOlogie et GEstion des Risques en agriculture
Tatiana Giraud - Ecologie Systématique et Evolution
Celedonio Gonzalez - Departamento de Bioquímica y Biología Molecular
Sandrine Grossetete - Microbiologie, adaptation et pathogénie
Ulrich Gueldener - Helmholtz Zentrum München = German Research Center for Environmental Health
Bernard Henrissat - Architecture et fonction des macromolécules biologiques
Barbara J. Howlett - School of Botany [Melbourne]
Chinnappa Kodira - Broad Institute of MIT and Harvard
Matthias Kretschmer - Faculty of Biology
Anne Lappartient - Microbiologie, adaptation et pathogénie
Michaela Leroch - Faculty of Biology
Caroline Levis - BIOlogie et GEstion des Risques en agriculture
Evan Mauceli - Broad Institute of MIT and Harvard
Cécile Neuvéglise - Microbiologie de l’alimentation au service de la santé
Birgitt Oeser - Molekularbiologie und Biotechnologie der Pilze
Matthew Pearson - Broad Institute of MIT and Harvard
Julie Poulain - Genoscope - Centre national de séquençage [Evry]
Nathalie Poussereau - Génomique fonctionnelle des champignons pathogènes des plantes
Hadi Quesneville - Unité de Recherche Génomique Info
Christine Rascle - Centre National de la Recherche Scientifique
Julia Schumacher - Molekularbiologie und Biotechnologie der Pilze
Béatrice Segurens - Genoscope - Centre national de séquençage [Evry]
Adrienne Sexton - School of Botany [Melbourne]
Evelyn Silva - Fundacion Ciencia para la Vida and Facultad de Ciencias Biologicas
Catherine Sirven - Microbiologie, adaptation et pathogénie
Darren M. Soanes - School of Biosciences
Nicholas J. Talbot - School of Biosciences
Matt Templeton - Plant and Food Research
Chandri Yandava - Broad Institute of MIT and Harvard
Oded Yarden - Department of Plant Pathology and Microbiology
Qiandong Zeng - Broad Institute of MIT and Harvard
Jeffrey A. Rollins - Department of Plant Pathology
Marc-Henri Lebrun - BIOlogie et GEstion des Risques en agriculture
Marty Dickman - Borlaug Genomics and Bioinformatics Center, Department of Plant Pathology and Microbiology
Détails de publication: PLoS genetics, Vol.7(8), p.e1002230
Éditeur: Public Library of Science
Identifiants: 99157236409311
Unité académique: Sciences pour l'oenologie - SPO; Plant Health Institute of Montpellier - PHIM
Langue: English
Type de ressource: Journal article