Genome-wide analysis of transcriptomic divergence between laboratory colony and field Anopheles gambiae mosquitoes of the M and S molecular forms

Ruth Aguilar; Frederic Simard; Colince Kamdem; Tim Shields; Gregory E. Glass; Lindsey S. Garver; George Dimopoulos

doi:10.1111/j.1365-2583.2010.01031.x

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Genome-wide analysis of transcriptomic divergence between laboratory colony and field Anopheles gambiae mosquitoes of the M and S molecular forms

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Genome-wide analysis of transcriptomic divergence between laboratory colony and field Anopheles gambiae mosquitoes of the M and S molecular forms

Ruth Aguilar, Frederic Simard, Colince Kamdem, Tim Shields, Gregory E. Glass, Lindsey S. Garver et George Dimopoulos

Insect molecular biology, Vol.19(5), pp.695-705

10/2010

DOI: https://doi.org/10.1111/j.1365-2583.2010.01031.x

PMID: 20738426

Résumé

Our knowledge of Anopheles gambiae molecular biology has mainly been based on studies using inbred laboratory strains. Differences in the environmental exposure of these and natural field mosquitoes have inevitably led to physiological divergences. We have used global transcript abundance analyses to probe into this divergence, and identified transcript abundance patterns of genes that provide insight on specific adaptations of caged and field mosquitoes. We also compared the gene transcript abundance profiles of field mosquitoes belonging to the two morphologically indistinguishable but reproductively isolated sympatric molecular forms, M and S, from two different locations in the Yaoundé area of Cameroon. This analysis suggested that environmental exposure has a greater influence on the transcriptome than does the mosquito’s molecular form-specific genetic background.

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Titre: Genome-wide analysis of transcriptomic divergence between laboratory colony and field Anopheles gambiae mosquitoes of the M and S molecular forms
Créateurs - sans rôle: Ruth Aguilar - W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, 615N. Wolfe Street, Baltimore, MD 21205-2179, USA Laboratoire de Recherche sur le Paludisme, OCEAC (Organisation de Coordination pour la lutte Contre les Endemies en Afrique Centrale), B.P. 288 Yaoundé, Cameroun Institut de Recherche pour le Développement (IRD), Research Unit #016, 911 Avenue Agropolis, 34 394 Montpellier, France
Frederic Simard - W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, 615N. Wolfe Street, Baltimore, MD 21205-2179, USA Laboratoire de Recherche sur le Paludisme, OCEAC (Organisation de Coordination pour la lutte Contre les Endemies en Afrique Centrale), B.P. 288 Yaoundé, Cameroun Institut de Recherche pour le Développement (IRD), Research Unit #016, 911 Avenue Agropolis, 34 394 Montpellier, France
Colince Kamdem - W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, 615N. Wolfe Street, Baltimore, MD 21205-2179, USA Laboratoire de Recherche sur le Paludisme, OCEAC (Organisation de Coordination pour la lutte Contre les Endemies en Afrique Centrale), B.P. 288 Yaoundé, Cameroun Institut de Recherche pour le Développement (IRD), Research Unit #016, 911 Avenue Agropolis, 34 394 Montpellier, France
Tim Shields - W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, 615N. Wolfe Street, Baltimore, MD 21205-2179, USA Laboratoire de Recherche sur le Paludisme, OCEAC (Organisation de Coordination pour la lutte Contre les Endemies en Afrique Centrale), B.P. 288 Yaoundé, Cameroun Institut de Recherche pour le Développement (IRD), Research Unit #016, 911 Avenue Agropolis, 34 394 Montpellier, France
Gregory E. Glass - W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, 615N. Wolfe Street, Baltimore, MD 21205-2179, USA Laboratoire de Recherche sur le Paludisme, OCEAC (Organisation de Coordination pour la lutte Contre les Endemies en Afrique Centrale), B.P. 288 Yaoundé, Cameroun Institut de Recherche pour le Développement (IRD), Research Unit #016, 911 Avenue Agropolis, 34 394 Montpellier, France
Lindsey S. Garver - W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, 615N. Wolfe Street, Baltimore, MD 21205-2179, USA Laboratoire de Recherche sur le Paludisme, OCEAC (Organisation de Coordination pour la lutte Contre les Endemies en Afrique Centrale), B.P. 288 Yaoundé, Cameroun Institut de Recherche pour le Développement (IRD), Research Unit #016, 911 Avenue Agropolis, 34 394 Montpellier, France
George Dimopoulos - W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, 615N. Wolfe Street, Baltimore, MD 21205-2179, USA Laboratoire de Recherche sur le Paludisme, OCEAC (Organisation de Coordination pour la lutte Contre les Endemies en Afrique Centrale), B.P. 288 Yaoundé, Cameroun Institut de Recherche pour le Développement (IRD), Research Unit #016, 911 Avenue Agropolis, 34 394 Montpellier, France
Détails de publication: Insect molecular biology, Vol.19(5), pp.695-705
Nombre de pages: 11
Identifiants: 99147945309311
Unité académique: Maladies Infectieuses et Vecteurs : Ecologie, Génétique, Evolution et Contrôle - MIVEGEC
Langue: English
Type de ressource: Journal article