Genetics of nodulation in Aeschynomene evenia uncovers mechanisms of the rhizobium-legume symbiosis

Johan Quilbé; Léo Lamy; Laurent Brottier; Philippe Leleux; Joël Fardoux; Ronan Rivallan; Thomas Benichou; Rémi Guyonnet; Manuel Becana; Irene Villar; Olivier Garsmeur; Bárbara Hufnagel; Amandine Delteil; Djamel Gully; Clémence Chaintreuil; Marjorie Pervent; Fabienne Cartieaux; Mickaël Bourge; Nicolas Valentin; Guillaume Martin; Loïc Fontaine; Gaëtan Droc; Alexis Dereeper; Andrew Farmer; Cyril Libourel; Nico Nouwen; Frédéric Gressent; Pierre Mournet; Angélique d'Hont; Eric Giraud; Christophe Klopp; Jean-François Arrighi

doi:10.1038/s41467-021-21094-7

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Genetics of nodulation in Aeschynomene evenia uncovers mechanisms of the rhizobium-legume symbiosis

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Genetics of nodulation in Aeschynomene evenia uncovers mechanisms of the rhizobium-legume symbiosis

Johan Quilbé, Léo Lamy, Laurent Brottier, Philippe Leleux, Joël Fardoux, Ronan Rivallan, Thomas Benichou, Rémi Guyonnet, Manuel Becana, Irene Villar, …

Nature Communications, Vol.12

2021

DOI: https://doi.org/10.1038/s41467-021-21094-7

PMID: 33547303

Abstract

Among legumes (Fabaceae) capable of nitrogen-fixing nodulation, several Aeschynomene spp. use a unique symbiotic process that is independent of Nod factors and infection threads. They are also distinctive in developing root and stem nodules with photosynthetic bradyrhizobia. Despite the significance of these symbiotic features, their understanding remains limited. To overcome such limitations, we conduct genetic studies of nodulation in Aeschynomene evenia, supported by the development of a genome sequence for A. evenia and transcriptomic resources for 10 additional Aeschynomene spp. Comparative analysis of symbiotic genes substantiates singular mechanisms in the early and late nodulation steps. A forward genetic screen also shows that AeCRK, coding a receptor-like kinase, and the symbiotic signaling genes AePOLLUX, AeCCamK, AeCYCLOPS, AeNSP2, and AeNIN are required to trigger both root and stem nodulation. This work demonstrates the utility of the A. evenia model and provides a cornerstone to unravel mechanisms underlying the rhizobium-legume symbiosis.

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Title: Genetics of nodulation in Aeschynomene evenia uncovers mechanisms of the rhizobium-legume symbiosis
Creators - without role: Johan Quilbé - Laboratoire des Symbioses Tropicales et Méditerranéennes
Léo Lamy - Laboratoire des Symbioses Tropicales et Méditerranéennes
Laurent Brottier - Laboratoire des Symbioses Tropicales et Méditerranéennes
Philippe Leleux - Laboratoire des Symbioses Tropicales et Méditerranéennes
Joël Fardoux - Laboratoire des Symbioses Tropicales et Méditerranéennes
Ronan Rivallan - Genetic Improvement and Adaptation of Mediterranean and Tropical Plants
Thomas Benichou - Laboratoire des Symbioses Tropicales et Méditerranéennes
Rémi Guyonnet - Laboratoire des Symbioses Tropicales et Méditerranéennes
Manuel Becana - Estación Experimental de Aula Dei
Irene Villar - Estación Experimental de Aula Dei
Olivier Garsmeur - Genetic Improvement and Adaptation of Mediterranean and Tropical Plants
Bárbara Hufnagel - Université de Montpellier, Institut des Sciences des Plantes de Montpellier - IPSiM
Amandine Delteil - Laboratoire des Symbioses Tropicales et Méditerranéennes
Djamel Gully - Laboratoire des Symbioses Tropicales et Méditerranéennes
Clémence Chaintreuil - Laboratoire des Symbioses Tropicales et Méditerranéennes
Marjorie Pervent - Laboratoire des Symbioses Tropicales et Méditerranéennes
Fabienne Cartieaux - Laboratoire des Symbioses Tropicales et Méditerranéennes
Mickaël Bourge - Institut de Biologie Intégrative de la Cellule
Nicolas Valentin - Institut de Biologie Intégrative de la Cellule
Guillaume Martin - Genetic Improvement and Adaptation of Mediterranean and Tropical Plants
Loïc Fontaine - Biologie et Génétique des Interactions Plante-Parasite
Gaëtan Droc - Agropolis International
Alexis Dereeper - Genetic Improvement and Adaptation of Mediterranean and Tropical Plants
Andrew Farmer - National Center for Genome Resources
Cyril Libourel - Laboratoire de Recherche en Sciences Végétales
Nico Nouwen - Laboratoire des Symbioses Tropicales et Méditerranéennes
Frédéric Gressent - Laboratoire des Symbioses Tropicales et Méditerranéennes
Pierre Mournet - Centre de Coopération Internationale en Recherche Agronomique pour le Développement
Angélique d'Hont - Amélioration génétique et adaptation des plantes méditerranéennes et tropicales
Eric Giraud - Laboratoire des Symbioses Tropicales et Méditerranéennes
Christophe Klopp - Unité de Biométrie et Intelligence Artificielle (ancêtre de MIAT)
Jean-François Arrighi - Laboratoire des Symbioses Tropicales et Méditerranéennes
Publication Details: Nature Communications, Vol.12
Grant note: Spanish Ministry of Science and Innovation-European Regional Development Fund (AGL2017-85775-R)
Identifiers: 9964233309311
Academic Unit: Plant Health Institute of Montpellier - PHIM; Institut des Sciences des Plantes de Montpellier - IPSiM
Language: English
Resource Type: Journal article
Local Fields: hal-03141624