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Freezing and storage at −20 °C provides adequate preservation of Toxoplasma gondii DNA for retrospective molecular analysis
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Freezing and storage at −20 °C provides adequate preservation of Toxoplasma gondii DNA for retrospective molecular analysis

Laurence Delhaes, Denis Filisetti, Marie-Pierre Brenier-Pinchart, Hervé Pelloux, Hélène Yera, Frédéric Dalle, Yvon Sterkers, Emmanuelle Varlet-Marie, Feriel Touafek, Sophie Cassaing, …
Diagnostic Microbiology and Infectious Disease, Vol.80(3), pp.197-199
11/2014
DOI: https://doi.org/10.1016/j.diagmicrobio.2014.08.007

Résumé

real-Time PCR DNA storage toxoplasma gondii congenital toxoplasmosis amniotic fluid DNA, Protozoan/isolation&purification; Freezing; Molecular Diagnostic Technics/methods; Specimen Handling/methods; Toxoplasmosis/diagnosis
Nucleic acid-based testing has become crucial for toxoplasmosis diagnosis. For retrospective (forensic or scientific) studies, optimal methods must be employed for DNA long-term storage. We compared Toxoplasma gondii detection before and after DNA storage using real-time PCR. No significant differences were found depending on duration or storage conditions at -20 °C or -80 °C.

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