Flow cytometric enumeration of bacterial in the coral surface mucus layer

Yvan Bettarel; Thanh Chi Mai; Patrice Got; Antoinette Adingra; Nadège Kouadio-Ngbesso; Bui van Ngoc; Thierry Bouvier

doi:10.1016/j.mimet.2016.05.032

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Flow cytometric enumeration of bacterial in the coral surface mucus layer

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Flow cytometric enumeration of bacterial in the coral surface mucus layer

Yvan Bettarel, Thanh Chi Mai, Patrice Got, Antoinette Adingra, Nadège Kouadio-Ngbesso, Bui van Ngoc et Thierry Bouvier

Journal of Microbiological Methods, Vol.128, pp.16 - 19

09/2016

DOI: https://doi.org/10.1016/j.mimet.2016.05.032

Résumé

Mucus

Microscopy

Fluorescent dyes

Epibionts

Corals

Bacteria

The direct counts of bacteria inhabiting coral mucus were performed by flow cytometry testing four fluorescent dyes (SYBR®Green I, HCS, TOPRO®3, SYTO®62) with three different scleractinian species. Results obtained with SYTO62 were the most reliable based on the comparison with standardized epifluorescence counts and the resolution of cytograms.

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Détails

Titre: Flow cytometric enumeration of bacterial in the coral surface mucus layer
Créateurs - sans rôle: Yvan Bettarel - Université de Montpellier, MARine Biodiversity, Exploitation & Conservation - MARBEC
Thanh Chi Mai
Patrice Got - Université de Montpellier, MARine Biodiversity, Exploitation & Conservation - MARBEC
Antoinette Adingra - Center for Marine and Oceanographic Research
Nadège Kouadio-Ngbesso
Bui van Ngoc
Thierry Bouvier - Université de Montpellier, MARine Biodiversity, Exploitation & Conservation - MARBEC
Détails de publication: Journal of Microbiological Methods, Vol.128, pp.16 - 19
Identifiants: 99152071909311
Unité académique: MARine Biodiversity, Exploitation & Conservation - MARBEC
Langue: English
Type de ressource: Journal article
Champs locaux: hal-01924093