Résumé
Prooligonucleotides with alternating S-Acyl-ThioEthyl (SATE) phosphotriester and phosphodiester linkages or a gap were synthesized by elimination of cyanoethyl group with non-nucleophilic base DBU. Their half-lives in the presence of Pig Liver Esterases (PLE) and in total cell extract were determined.
Prooligonucleotides with an alternating SATE phosphotriester and phosphodiester or a gap were synthesized by elimination of cyanoethyl groups with non-nucleophilic base DBU in dry THF. Their half-lives in the presence of Pig Liver Esterases and in total cell extract were determined.