Résumé
Background and AimsThe aim of this work was to establish a general method for evaluating the intrinsic activity of yeasts towards the release of volatiles terpenes from glycosidic aroma precursors. Methods and ResultsThe enzymatic capacity of yeast cells towards glycosidic aroma precursors was assessed on permeabilised yeast cells. In this way, the pores created into the cell membrane allowed the precursors to freely enter the cells. ConclusionsThe comparison between the release of terpenes from glycosidic aroma precursors by entire viable cells during fermentation and by permeabilised cells shows that the permeation of glycosidic aroma precursors through the yeast cell membrane is the main bottleneck for such release. Significance of the StudyThis work highlights the fact that Saccharomyces species can exhibit -glycosidase activity as high as non-Saccharomyces species. This important result points out future prospects for the improvement of Saccharomyces species by classical genetics.