Résumé
Background: Until now, specific proteins have traditionally been analyzed by immunonephelometry requiring specific equipment. Due to laboratory consolidation, turbidimetry is commonly used for the determination of specific proteins. Biochemistry analyzers have a wide range of turbidimetric assays available to a single workstation, which makes them particularly efficient. For our laboratory consolidation, we installed two distinct Cobas8000 (R) modular analyzer series. However, the turbidimetric albumin from Roche did not give satisfactory results in regard to the traceability to the reference measurement material.
Methods: Analytical assays of turbidimetric albumin from Diagam (Liege, Belgique) on c502/Cobas8000 (R), including imprecision studies, accuracy using the ERM (R)-DA470k/IFCC, and correlation with nephelometry and colorimetry, were performed.
Results: Total precision for the immunoturbidimetric assays was consistently better than 2.3% CV and linear throughout the dynamic range of the assays. Correlation of serum albumin by turbidimetry is in good agreement with nephelometry. The mean +/- SD of the reference material ERM-DA470k was 37.6 +/- 0.25 g/L, with a bias of 1.07%.
Conclusions: Turbidimetric albumin from Diagam meets the requirements of accuracy and precision for optimal clinical use and could be an alternative without the associated cost of a dedicated instrument in the context of laboratory consolidation.