Estimation of HIV-1 DNA Level Interfering with Reliability of HIV-1 RNA Quantification Performed on Dried Blood Spots Collected from Successfully Treated Patients

Sylvie Zida; Edouard Tuaillon; Makoura Barro; Arnaud Kwimatouo Lekpa Franchard; Thérèse Kagoné; Boubacar Nacro; Abdoul Salam Ouedraogo; Karine Bolloré; Armen Sanosyan; Jean-Christophe Plantier; Nicolas Meda; Lassana Sangare; Christine Rouzioux; François Rouet; Dramane Kania

doi:10.1128/JCM.03372-15

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Estimation of HIV-1 DNA Level Interfering with Reliability of HIV-1 RNA Quantification Performed on Dried Blood Spots Collected from Successfully Treated Patients

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Estimation of HIV-1 DNA Level Interfering with Reliability of HIV-1 RNA Quantification Performed on Dried Blood Spots Collected from Successfully Treated Patients

Sylvie Zida, Edouard Tuaillon, Makoura Barro, Arnaud Kwimatouo Lekpa Franchard, Thérèse Kagoné, Boubacar Nacro, Abdoul Salam Ouedraogo, Karine Bolloré, Armen Sanosyan, Jean-Christophe Plantier, …

Journal of Clinical Microbiology, Vol.54(6), pp.1641 - 1643

06/2016

DOI: https://doi.org/10.1128/JCM.03372-15

PMCID: PMC4879270

PMID: 27008874

Abstract

The impact of HIV-1 DNA coamplification during HIV-1 RNA quantification on dried blood spots (DBS) was explored. False-positive HIV RNA detection (22/62, 35%) was associated with high HIV-1 DNA levels. Specificity of HIV-1 RNA assays on DBS should be evaluated following manufacturer protocols on samples with HIV-1 DNA levels of ≥1,000 copies/10(6) peripheral blood mononuclear cells.

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Title: Estimation of HIV-1 DNA Level Interfering with Reliability of HIV-1 RNA Quantification Performed on Dried Blood Spots Collected from Successfully Treated Patients
Creators - without role: Sylvie Zida - Université de Montpellier, Pathogenesis and Control of Chronic and Emerging Infections - PCCEI
Edouard Tuaillon - Université de Montpellier, Pathogenesis and Control of Chronic and Emerging Infections - PCCEI
Makoura Barro - Nazi Boni University
Arnaud Kwimatouo Lekpa Franchard - Centre Muraz
Thérèse Kagoné - Centre Muraz
Boubacar Nacro - Nazi Boni University
Abdoul Salam Ouedraogo - Centre Hospitalier Universitaire Yalgado Ouédraogo
Karine Bolloré - Université de Montpellier, Pathogenesis and Control of Chronic and Emerging Infections - PCCEI
Armen Sanosyan - Université de Montpellier, Pathogenesis and Control of Chronic and Emerging Infections - PCCEI
Jean-Christophe Plantier - Virologie
Nicolas Meda - Centre Muraz
Lassana Sangare - Centre Hospitalier Universitaire Yalgado Ouédraogo
Christine Rouzioux - Délégation Paris 5
François Rouet - Institut Pasteur du Cambodge
Dramane Kania - Centre Muraz
Publication Details: Journal of Clinical Microbiology, Vol.54(6), pp.1641 - 1643
Identifiers: 9938886909311
Academic Unit: Pathogenesis and Control of Chronic and Emerging Infections - PCCEI
Language: English
Resource Type: Journal article
Local Fields: hal-01872543