Résumé
Silica monoliths with uniform macro-/mesoporous structures (20 µm and 20 nm macro-and mesopores diameters, respectively), high porosity (83%) and high surface area (370 m 2 g-1) were prepared. The monoliths were grafted with amino groups (0.9 mmol NH 2 g-1) and used to immobilize laccase from Trametes versicolor by covalent grafting with glutaraldehyde (GLU) (1.0 mmol GLU g-1) leading to an ABTS activity of 20 U g-1. Immobilization yield was 80%, based on the difference of initial and final activity of enzymatic solution used for immobilization. Enzymatic monoliths were used for the degradation of tetracycline (TC) in aqueous solution (20 mg L-1) in continuous flow with recycling configuration. TC degradation efficiency was found to be 40-50 % after 5 h of reaction at pH 7. Enzymatic monoliths were used during 75 hours of sequential operation without losing activity. A Steady-state computational fluid dynamics (CFD) model based on Michaelis Menten reaction kinetics, allowed computing TC degradation efficiency.