Résumé
1.
1.
[
3
H]N-[1-(2-
Benzo(b)thiophenyl)cyclohexyl]piperidine
([
3H]BTCP) labels
in vivo the dopamine uptake complex in the mouse striatum.
2.
2. In mice treated with γ-butyrolactone (GBL, 400 mg/kg), [
3H]BTCP specific binding was increased and ID
50 values of BTCP and cocaine for the prevention of [
3H]BTCP binding were significantly lowered.
3.
3. In GBL-treated mice, cocaine (5 mg/kg, s.c.) had no effect on the BTCP ID
50 value, whereas GBR 12783 (2 mg/kg, s.c.) increased it significantly.
4.
4. Thus
in vivo, endogenous dopamine and cocaine are competitive and non-competitive inhibitors, respectively, of the binding of [
3H]BTCP.