Dynamics of Gene Introgression in the African Malaria Vector Anopheles gambiae

Daibin Zhong; Emmanuel A. Temu; Tom Guda; Louis Gouagna; David Menge; Aditi Pai; John Githure; John C. Beier; Guiyun Yan

doi:10.1534/genetics.105.050781

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Dynamics of Gene Introgression in the African Malaria Vector Anopheles gambiae

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Dynamics of Gene Introgression in the African Malaria Vector Anopheles gambiae

Daibin Zhong, Emmanuel A. Temu, Tom Guda, Louis Gouagna, David Menge, Aditi Pai, John Githure, John C. Beier et Guiyun Yan

Genetics (Austin), Vol.172(4), pp.2359-2365

01/04/2006

DOI: https://doi.org/10.1534/genetics.105.050781

PMID: 16452145

Résumé

Investigations

Anopheles gambiae is a major malaria vector in Africa and a popular model species for a variety of ecological, evolutionary, and genetic studies on vector control. Genetic manipulation of mosquito vectorial capacity is a promising new weapon for the control of malaria. However, the release of exotic transgenic mosquitoes will bring in novel alleles in addition to the parasite-inhibiting genes, which may have unknown effects on the local population. Therefore, it is necessary to develop methodologies that can be used to evaluate the spread rate of introduced genes in A. gambiae. In this study, the effects and dynamics of genetic introgression between two geographically distinct A. gambiae populations from western Kenya (Mbita) and eastern Tanzania (Ifakara) were investigated with amplified fragment length polymorphisms (AFLPs) and microsatellite markers. Microsatellites and polymorphic cDNA markers revealed a large genetic differentiation between the two populations (average FST = 0.093, P < 0.001). When the two strains were crossed in random mating between the two populations, significant differences in the rate of genetic introgression were found in the mixed populations. Allele frequencies of 18 AFLP markers (64.3%) for Mbita and of 26 markers (92.9%) for Ifakara varied significantly from F5 to F20. This study provides basic information on how a mosquito release program would alter the genetic makeup of natural populations, which is critical for pilot field testing and ecological risk evaluation of transgenic mosquitoes.

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Détails

Titre: Dynamics of Gene Introgression in the African Malaria Vector Anopheles gambiae
Créateurs - sans rôle: Daibin Zhong - Program in Public Health, College of Health Sciences, University of California, Irvine, California 92697, Department of Biological Sciences, State University of New York, Buffalo, New York 14260, Mbita Point Field and Training Station, International Center of Insect Physiology and Ecology, Nairobi, Kenya and Department of Epidemiology and Public Health, University of Miami, Miami, Florida 33177
Emmanuel A. Temu - Program in Public Health, College of Health Sciences, University of California, Irvine, California 92697, Department of Biological Sciences, State University of New York, Buffalo, New York 14260, Mbita Point Field and Training Station, International Center of Insect Physiology and Ecology, Nairobi, Kenya and Department of Epidemiology and Public Health, University of Miami, Miami, Florida 33177
Tom Guda - Program in Public Health, College of Health Sciences, University of California, Irvine, California 92697, Department of Biological Sciences, State University of New York, Buffalo, New York 14260, Mbita Point Field and Training Station, International Center of Insect Physiology and Ecology, Nairobi, Kenya and Department of Epidemiology and Public Health, University of Miami, Miami, Florida 33177
Louis Gouagna - Program in Public Health, College of Health Sciences, University of California, Irvine, California 92697, Department of Biological Sciences, State University of New York, Buffalo, New York 14260, Mbita Point Field and Training Station, International Center of Insect Physiology and Ecology, Nairobi, Kenya and Department of Epidemiology and Public Health, University of Miami, Miami, Florida 33177
David Menge - Program in Public Health, College of Health Sciences, University of California, Irvine, California 92697, Department of Biological Sciences, State University of New York, Buffalo, New York 14260, Mbita Point Field and Training Station, International Center of Insect Physiology and Ecology, Nairobi, Kenya and Department of Epidemiology and Public Health, University of Miami, Miami, Florida 33177
Aditi Pai - Program in Public Health, College of Health Sciences, University of California, Irvine, California 92697, Department of Biological Sciences, State University of New York, Buffalo, New York 14260, Mbita Point Field and Training Station, International Center of Insect Physiology and Ecology, Nairobi, Kenya and Department of Epidemiology and Public Health, University of Miami, Miami, Florida 33177
John Githure - Program in Public Health, College of Health Sciences, University of California, Irvine, California 92697, Department of Biological Sciences, State University of New York, Buffalo, New York 14260, Mbita Point Field and Training Station, International Center of Insect Physiology and Ecology, Nairobi, Kenya and Department of Epidemiology and Public Health, University of Miami, Miami, Florida 33177
John C. Beier - Program in Public Health, College of Health Sciences, University of California, Irvine, California 92697, Department of Biological Sciences, State University of New York, Buffalo, New York 14260, Mbita Point Field and Training Station, International Center of Insect Physiology and Ecology, Nairobi, Kenya and Department of Epidemiology and Public Health, University of Miami, Miami, Florida 33177
Guiyun Yan - University of California, Irvine
Détails de publication: Genetics (Austin), Vol.172(4), pp.2359-2365
Nombre de pages: 7
Identifiants: 99147908509311
Unité académique: Maladies Infectieuses et Vecteurs : Ecologie, Génétique, Evolution et Contrôle - MIVEGEC
Langue: English
Type de ressource: Journal article