Résumé
Phytosterols and steryl ferulates are bioactive compounds accumulating in the bran and germ of wheat. However, little is known regarding their localisation and composition in the bran layers of the kernel. The aim of this study was to determine the distribution of phytosterols and steryl ferulates in the wheat grain and in the different layers of bran. The wheat fractions, produced by conventional debranning, aleurone separation and a novel electrostatic process, were analysed for phytosterol contents using GC-FID and for steryl ferulate contents using HPLC-UV. The compounds were identified by GC- and LC-MS. Phytosterols and steryl ferulates were concentrated in the bran layers. The steryl ferulates were accumulated in the intermediate layers, whereas the phytosterols were more evenly distributed in the intermediate layers and aleurone cell contents. The phytosterol composition varied within the wheat kernel, while the steryl ferulate composition was similar in different fractions. Sitosterol and campestanyl ferulate were the main compounds. The highest levels of phytosterols (up to 2117 mu g/g) and steryl ferulates (up to 703 mu g) were found in the pearling, aleurone and certain bran fractions. The phytosterol-rich fractions could be utilised in cereal foods to enhance the intake of health-promoting compounds from natural sources. (c) 2012 Elsevier Ltd. All rights reserved.