Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora

Pierre Marraccini; Felipe Vinecky; Gabriel S.C. Alves; Humberto J.O. Ramos; Sonia Elbelt; Natalia G. Vieira; Fernanda A. Carneiro; Patricia S. Sujii; Jean C. Alekcevetch; Vânia A. Silva; Fábio M. DaMatta; Maria A.G. Ferrão; Thierry Leroy; David Pot; Luiz G.E. Vieira; Felipe R. da Silva; Alan C. Andrade

doi:10.1093/jxb/ers103

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Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora

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Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora

Pierre Marraccini, Felipe Vinecky, Gabriel S.C. Alves, Humberto J.O. Ramos, Sonia Elbelt, Natalia G. Vieira, Fernanda A. Carneiro, Patricia S. Sujii, Jean C. Alekcevetch, Vânia A. Silva, …

Journal of experimental botany, Vol.63(11), pp.4191-4212

01/06/2012

DOI: https://doi.org/10.1093/jxb/ers103

PMID: 22511801

Résumé

Research Paper

The aim of this study was to investigate the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes (CGs) using different approaches. The first approach used the data generated during the Brazilian Coffee expressed sequence tag (EST) project to select 13 CGs by an in silico analysis (electronic northern). The second approach was based on screening macroarrays spotted with plasmid DNA (coffee ESTs) with separate hybridizations using leaf cDNA probes from drought-tolerant and susceptible clones of Coffea canephora var. Conilon, grown under different water regimes. This allowed the isolation of seven additional CGs. The third approach used two-dimensional gel electrophoresis to identify proteins displaying differential accumulation in leaves of drought-tolerant and susceptible clones of C. canephora. Six of them were characterized by MALDI-TOF-MS/MS (matrix-assisted laser desorption-time of flight-tandem mass spectrometry) and the corresponding proteins were identified. Finally, additional CGs were selected from the literature, and quantitative real-time polymerase chain reaction (qPCR) was performed to analyse the expression of all identified CGs. Altogether, >40 genes presenting differential gene expression during drought acclimation were identified, some of them showing different expression profiles between drought-tolerant and susceptible clones. Based on the obtained results, it can be concluded that factors involved a complex network of responses probably involving the abscisic signalling pathway and nitric oxide are major molecular determinants that might explain the better efficiency in controlling stomata closure and transpiration displayed by drought-tolerant clones of C. canephora.

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Titre: Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora
Créateurs - sans rôle: Pierre Marraccini - Agropolis International
Felipe Vinecky - EMBRAPA Recursos Genéticos e Biotecnologia (LGM), Parque EB, CP 02372, 70770-917 Brasilia, DF, Brazil CIRAD, UMR AGAP, Avenue d’Agropolis, F 34398 Montpellier, France IAPAR, LBI-AMG, CP 481, 86001-970 Londrina, PR, Brazil UFV, Departamento de Biologia Vegetal, 36570-000 Viçosa, MG, Brazil INCAPER/EMBRAPA CAFÉ, Rod. BR 363, km 94, 29375-000 Domingos Martins, ES, Brazil
Gabriel S.C. Alves - EMBRAPA Recursos Genéticos e Biotecnologia (LGM), Parque EB, CP 02372, 70770-917 Brasilia, DF, Brazil CIRAD, UMR AGAP, Avenue d’Agropolis, F 34398 Montpellier, France IAPAR, LBI-AMG, CP 481, 86001-970 Londrina, PR, Brazil UFV, Departamento de Biologia Vegetal, 36570-000 Viçosa, MG, Brazil INCAPER/EMBRAPA CAFÉ, Rod. BR 363, km 94, 29375-000 Domingos Martins, ES, Brazil
Humberto J.O. Ramos - EMBRAPA Recursos Genéticos e Biotecnologia (LGM), Parque EB, CP 02372, 70770-917 Brasilia, DF, Brazil CIRAD, UMR AGAP, Avenue d’Agropolis, F 34398 Montpellier, France IAPAR, LBI-AMG, CP 481, 86001-970 Londrina, PR, Brazil UFV, Departamento de Biologia Vegetal, 36570-000 Viçosa, MG, Brazil INCAPER/EMBRAPA CAFÉ, Rod. BR 363, km 94, 29375-000 Domingos Martins, ES, Brazil
Sonia Elbelt - EMBRAPA Recursos Genéticos e Biotecnologia (LGM), Parque EB, CP 02372, 70770-917 Brasilia, DF, Brazil CIRAD, UMR AGAP, Avenue d’Agropolis, F 34398 Montpellier, France IAPAR, LBI-AMG, CP 481, 86001-970 Londrina, PR, Brazil UFV, Departamento de Biologia Vegetal, 36570-000 Viçosa, MG, Brazil INCAPER/EMBRAPA CAFÉ, Rod. BR 363, km 94, 29375-000 Domingos Martins, ES, Brazil
Natalia G. Vieira - EMBRAPA Recursos Genéticos e Biotecnologia (LGM), Parque EB, CP 02372, 70770-917 Brasilia, DF, Brazil CIRAD, UMR AGAP, Avenue d’Agropolis, F 34398 Montpellier, France IAPAR, LBI-AMG, CP 481, 86001-970 Londrina, PR, Brazil UFV, Departamento de Biologia Vegetal, 36570-000 Viçosa, MG, Brazil INCAPER/EMBRAPA CAFÉ, Rod. BR 363, km 94, 29375-000 Domingos Martins, ES, Brazil
Fernanda A. Carneiro - EMBRAPA Recursos Genéticos e Biotecnologia (LGM), Parque EB, CP 02372, 70770-917 Brasilia, DF, Brazil CIRAD, UMR AGAP, Avenue d’Agropolis, F 34398 Montpellier, France IAPAR, LBI-AMG, CP 481, 86001-970 Londrina, PR, Brazil UFV, Departamento de Biologia Vegetal, 36570-000 Viçosa, MG, Brazil INCAPER/EMBRAPA CAFÉ, Rod. BR 363, km 94, 29375-000 Domingos Martins, ES, Brazil
Patricia S. Sujii - EMBRAPA Recursos Genéticos e Biotecnologia (LGM), Parque EB, CP 02372, 70770-917 Brasilia, DF, Brazil CIRAD, UMR AGAP, Avenue d’Agropolis, F 34398 Montpellier, France IAPAR, LBI-AMG, CP 481, 86001-970 Londrina, PR, Brazil UFV, Departamento de Biologia Vegetal, 36570-000 Viçosa, MG, Brazil INCAPER/EMBRAPA CAFÉ, Rod. BR 363, km 94, 29375-000 Domingos Martins, ES, Brazil
Jean C. Alekcevetch - EMBRAPA Recursos Genéticos e Biotecnologia (LGM), Parque EB, CP 02372, 70770-917 Brasilia, DF, Brazil CIRAD, UMR AGAP, Avenue d’Agropolis, F 34398 Montpellier, France IAPAR, LBI-AMG, CP 481, 86001-970 Londrina, PR, Brazil UFV, Departamento de Biologia Vegetal, 36570-000 Viçosa, MG, Brazil INCAPER/EMBRAPA CAFÉ, Rod. BR 363, km 94, 29375-000 Domingos Martins, ES, Brazil
Vânia A. Silva - EMBRAPA Recursos Genéticos e Biotecnologia (LGM), Parque EB, CP 02372, 70770-917 Brasilia, DF, Brazil CIRAD, UMR AGAP, Avenue d’Agropolis, F 34398 Montpellier, France IAPAR, LBI-AMG, CP 481, 86001-970 Londrina, PR, Brazil UFV, Departamento de Biologia Vegetal, 36570-000 Viçosa, MG, Brazil INCAPER/EMBRAPA CAFÉ, Rod. BR 363, km 94, 29375-000 Domingos Martins, ES, Brazil
Fábio M. DaMatta - EMBRAPA Recursos Genéticos e Biotecnologia (LGM), Parque EB, CP 02372, 70770-917 Brasilia, DF, Brazil CIRAD, UMR AGAP, Avenue d’Agropolis, F 34398 Montpellier, France IAPAR, LBI-AMG, CP 481, 86001-970 Londrina, PR, Brazil UFV, Departamento de Biologia Vegetal, 36570-000 Viçosa, MG, Brazil INCAPER/EMBRAPA CAFÉ, Rod. BR 363, km 94, 29375-000 Domingos Martins, ES, Brazil
Maria A.G. Ferrão - EMBRAPA Recursos Genéticos e Biotecnologia (LGM), Parque EB, CP 02372, 70770-917 Brasilia, DF, Brazil CIRAD, UMR AGAP, Avenue d’Agropolis, F 34398 Montpellier, France IAPAR, LBI-AMG, CP 481, 86001-970 Londrina, PR, Brazil UFV, Departamento de Biologia Vegetal, 36570-000 Viçosa, MG, Brazil INCAPER/EMBRAPA CAFÉ, Rod. BR 363, km 94, 29375-000 Domingos Martins, ES, Brazil
Thierry Leroy - Agropolis International
David Pot - Agropolis International
Luiz G.E. Vieira - EMBRAPA Recursos Genéticos e Biotecnologia (LGM), Parque EB, CP 02372, 70770-917 Brasilia, DF, Brazil CIRAD, UMR AGAP, Avenue d’Agropolis, F 34398 Montpellier, France IAPAR, LBI-AMG, CP 481, 86001-970 Londrina, PR, Brazil UFV, Departamento de Biologia Vegetal, 36570-000 Viçosa, MG, Brazil INCAPER/EMBRAPA CAFÉ, Rod. BR 363, km 94, 29375-000 Domingos Martins, ES, Brazil
Felipe R. da Silva - EMBRAPA Recursos Genéticos e Biotecnologia (LGM), Parque EB, CP 02372, 70770-917 Brasilia, DF, Brazil CIRAD, UMR AGAP, Avenue d’Agropolis, F 34398 Montpellier, France IAPAR, LBI-AMG, CP 481, 86001-970 Londrina, PR, Brazil UFV, Departamento de Biologia Vegetal, 36570-000 Viçosa, MG, Brazil INCAPER/EMBRAPA CAFÉ, Rod. BR 363, km 94, 29375-000 Domingos Martins, ES, Brazil
Alan C. Andrade - EMBRAPA Recursos Genéticos e Biotecnologia (LGM), Parque EB, CP 02372, 70770-917 Brasilia, DF, Brazil CIRAD, UMR AGAP, Avenue d’Agropolis, F 34398 Montpellier, France IAPAR, LBI-AMG, CP 481, 86001-970 Londrina, PR, Brazil UFV, Departamento de Biologia Vegetal, 36570-000 Viçosa, MG, Brazil INCAPER/EMBRAPA CAFÉ, Rod. BR 363, km 94, 29375-000 Domingos Martins, ES, Brazil
Détails de publication: Journal of experimental botany, Vol.63(11), pp.4191-4212
Éditeur: Oxford University Press
Nombre de pages: 22
Identifiants: 99149720709311
Unité académique: Institut Amélioration Génétique & Adaptation des Plantes méditerranéennes & tropicales-AGAP; Diversité, Adaptation et Développement des plantes - DIADE
Langue: English
Type de ressource: Journal article