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Development of microsatellite-enriched libraries
Article de revue

Development of microsatellite-enriched libraries

Hélène Vignes et Ronan Rivallan
Methods in molecular biology (Clifton, N.J.), Vol.1115, pp.177-189
01/01/2014
PMID: 24415475

Résumé

Cloning, Molecular Dinucleotide Repeats - genetics DNA, Plant - genetics Genetic Loci - genetics Genome, Plant - genetics Genomic Library Microsatellite Repeats - genetics Oligonucleotide Probes - genetics Plasmids - genetics Polymerase Chain Reaction Sequence Analysis, DNA Transformation, Genetic
Among the molecular markers used for plant genetic studies, microsatellite markers are easy to implement and can provide suitable codominant markers for molecular taxonomy. Here we describe a method to obtain enriched libraries in microsatellite loci from genomic DNA, using capture method with synthetic oligonucleotide probes.

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