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Development of a polymerase chain reaction-restriction fragment length polymorphism assay for Leishmania major/ Leishmania killicki/ Leishmania infantum discrimination from clinical samples, application in a Tunisian focus
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Development of a polymerase chain reaction-restriction fragment length polymorphism assay for Leishmania major/ Leishmania killicki/ Leishmania infantum discrimination from clinical samples, application in a Tunisian focus

Najoua Haouas, Selma Garrab, Mohamed Gorcii, Hassen Khorchani, Najla Chargui, Christophe Ravel, Habib Mezhoud et Hamouda Babba
Diagnostic microbiology and infectious disease, Vol.68(2), pp.152-158
01/10/2010
DOI: https://doi.org/10.1016/j.diagmicrobio.2010.06.011
PMID: 20846587

Résumé

Leishmaniasis PCR-RFLP Species identification
Topoisomerase II gene of Leishmania genus was used to develop a molecular tool for detection and species differentiation of Leishmania from clinical samples. Identification was achieved by a polymerase chain reaction followed by digestion with 2 restriction endonucleases BstU1 and Taq1. Despite the relatively low sensitivity, it is able to differentiate between 3 complexes responsible for cutaneous leishmaniasis.

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