Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi

Matthew C. Fisher; Pria Ghosh; Jennifer M. G. Shelton; Kieran Bates; Lola Brookes; Claudia Wierzbicki; Goncalo M. Rosa; Rhys A. Farrer; David M. Aanensen; Mario Alvarado-Rybak; Arnaud Bataille; Lee Berger; Susanne Boell; Jaime Bosch; Frances C. Clare; Elodie A Courtois; Angelica Crottini; Andrew A. Cunningham; Thomas M. Doherty-Bone; Fikirte Gebresenbet; David J. Gower; Jacob Höglund; Timothy Y. James; Thomas S. Jenkinson; Tiffany A. Kosch; Carolina Lambertini; Anssi Laurila; Chun-Fu Lin; Adeline Loyau; An Martel; Sara Meurling; Claude Miaud; Pete Minting; Serge Ndriantsoa; Simon J. O'Hanlon; Frank Pasmans; Tsanta Rakotonanahary; Falitiana C. E. Rabemananjara; Luisa P. Ribeiro; Dirk S Schmeller; Benedikt R. Schmidt; Lee Skerratt; Freya Smith; Claudio Soto-Azat; Giulia Tessa; Luis Felipe Toledo; Andres Valenzuela-Sanchez; Ruhan Verster; Judit Voeroes; Bruce Waldman; Rebecca J. Webb; Che Weldon; Emma Wombwell; Kelly R. Zamudio; Joyce E. Longcore; Trenton W. J. Garner

doi:10.1038/s41598-018-24472-2

Retour

Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi

Article de revue

Avec comité de lecture

Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi

Matthew C. Fisher, Pria Ghosh, Jennifer M. G. Shelton, Kieran Bates, Lola Brookes, Claudia Wierzbicki, Goncalo M. Rosa, Rhys A. Farrer, David M. Aanensen, Mario Alvarado-Rybak, …

Scientific Reports, Vol.8

2018

DOI: https://doi.org/10.1038/s41598-018-24472-2

PMID: 29773857

Résumé

Parasitic chytrid fungi have emerged as a significant threat to amphibian species worldwide, necessitating the development of techniques to isolate these pathogens into culture for research purposes. However, early methods of isolating chytrids from their hosts relied on killing amphibians. We modified a pre-existing protocol for isolating chytrids from infected animals to use toe clips and biopsies from toe webbing rather than euthanizing hosts, and distributed the protocol to researchers as part of the BiodivERsA project RACE; here called the RML protocol. In tandem, we developed a lethal procedure for isolating chytrids from tadpole mouthparts. Reviewing a database of use a decade after their inception, we find that these methods have been applied across 5 continents, 23 countries and in 62 amphibian species. Isolation of chytrids by the non-lethal RML protocol occured in 18% of attempts with 207 fungal isolates and three species of chytrid being recovered. Isolation of chytrids from tadpoles occured in 43% of attempts with 334 fungal isolates of one species (Batrachochytrium dendrobatidis) being recovered. Together, these methods have resulted in a significant reduction and refinement of our use of threatened amphibian species and have improved our ability to work with this group of emerging pathogens.

Fichiers et liens (2)

url

Find in HALAfficher

url

https://doi.org/10.1038/s41598-018-24472-2Afficher

Published (Version of record) Ouvrir

Indicateurs

1 Consultations de la notice

See more details

Détails

Titre: Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi
Créateurs - sans rôle: Matthew C. Fisher - Imperial College London
Pria Ghosh - Imperial College London
Jennifer M. G. Shelton - Imperial College London
Kieran Bates - Imperial College London
Lola Brookes - Zoological Society of London
Claudia Wierzbicki - Imperial College London
Goncalo M. Rosa - University of Lisbon
Rhys A. Farrer - Imperial College London
David M. Aanensen - Imperial College London
Mario Alvarado-Rybak - Universidad Andrés Bello
Arnaud Bataille - Animal, Santé, Territoires, Risques et Ecosystèmes
Lee Berger - James Cook University
Susanne Boell - Agency for Population Ecology and Nature Conservancy
Jaime Bosch - Museo Nacional de Ciencias Naturales
Frances C. Clare - Imperial College London
Elodie A Courtois - University of French Guiana
Angelica Crottini - Universidade do Porto
Andrew A. Cunningham - Zoological Society of London
Thomas M. Doherty-Bone - Royal Zoological Society of Scotland
Fikirte Gebresenbet - Oklahoma State University
David J. Gower - Natural History Museum
Jacob Höglund - Department of Ecology and Genetics [Uppsala]
Timothy Y. James - University of Michigan
Thomas S. Jenkinson - University of Michigan
Tiffany A. Kosch - James Cook University
Carolina Lambertini - Universidade Estadual de Campinas (UNICAMP)
Anssi Laurila - Uppsala University
Chun-Fu Lin - Endemic Species Research Institute
Adeline Loyau - Université Toulouse III - Paul Sabatier
An Martel - Ghent University
Sara Meurling - Uppsala University
Claude Miaud - Université de Montpellier, Centre d'Ecologie Fonctionnelle et Evolutive - CEFE
Pete Minting - Amphibian and Reptile Conservation
Serge Ndriantsoa - Durrell Wildlife Conservation Trust
Simon J. O'Hanlon - Imperial College London
Frank Pasmans - Ghent University
Tsanta Rakotonanahary - Durrell Wildlife Conservation Trust
Falitiana C. E. Rabemananjara - Durrell Wildlife Conservation Trust
Luisa P. Ribeiro - Universidade Estadual de Campinas (UNICAMP)
Dirk S Schmeller - Institut National Polytechnique de Toulouse
Benedikt R. Schmidt - University of Zurich
Lee Skerratt - James Cook University
Freya Smith - Animal and Plant Health Agency
Claudio Soto-Azat - Universidad Andrés Bello
Giulia Tessa - Non-profit Association Zirichiltaggi - Sardinia Wildlife Conservation
Luis Felipe Toledo - Universidade Estadual de Campinas (UNICAMP)
Andres Valenzuela-Sanchez - Universidad Andrés Bello
Ruhan Verster - North-West University
Judit Voeroes - Hungarian Natural History Museum
Bruce Waldman - Seoul National University
Rebecca J. Webb - James Cook University
Che Weldon - North-West University
Emma Wombwell - Zoological Society of London
Kelly R. Zamudio - Cornell University
Joyce E. Longcore - University of Maine
Trenton W. J. Garner - Zoological Society of London
Détails de publication: Scientific Reports, Vol.8
Identifiants: 99161620809311
Unité académique: Centre d'Ecologie Fonctionnelle et Evolutive - CEFE
Langue: English
Type de ressource: Journal article
Champs locaux: hal-02625441