Résumé
- The objective of this work was to investigate the causes of sterility in a new set of triploid banana (Musa spp.) cultivars and to assess the chances of obtaining some progenies by manual cross-pollination. The developmental stages of female gametophyte were histologically recorded in ovules of four distinct triploid banana cultivars. Samples were taken on the day of flower opening and pollination and three days later. Morphologically mature embryo sacs were very rare in the two clones of the 'Cavendish' subgroup and in 'Prata Ana'. In 'Mysore', it occurred at a variable and low frequency. In 'Terrinha', it was extremely common. In the last two cases, the embryo sac maturation process was clearly continuing between the sampling days of flower opening and three days later. No pollen tube was positively identified in pollinated flowers within the integuments of any ovule of the older explants. Different proportions of mature embryo sacs are observed among the studied triploid genotypes of banana. The presence of mature embryo sacs is not an indication of functional capacity. Other causes of infertility in cultivated bananas can be due to some event immediately before or after fertilization, for instance, pollination tube growth failure or seed abortion.