Detection of enzyme activity at trace levels: A new perspective for the direct screening of active catalytic antibodies

T.D. Hua; Valérie Rolland; René Lazaro; P. Viallefont; M-P. Lefranc; M. Weill

doi:10.1016/0040-4039(95)02122-1

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Detection of enzyme activity at trace levels: A new perspective for the direct screening of active catalytic antibodies

Journal article

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Detection of enzyme activity at trace levels: A new perspective for the direct screening of active catalytic antibodies

T.D. Hua, Valérie Rolland, René Lazaro, P. Viallefont, M-P. Lefranc and M. Weill

Tetrahedron Letters, Vol.37(2), pp.175-178

1996

DOI: https://doi.org/10.1016/0040-4039(95)02122-1

PMID: 10707767

Abstract

catalytic antibodies

Detection

PCR

A very low level of enzyme activity (10−11 mol) has been detected using a PCR method following a vector hybridisation of a supported oligonucleotide. This methodology could be very useful for direct active catalytic antibody screening from a combinatorial library.

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Title: Detection of enzyme activity at trace levels: A new perspective for the direct screening of active catalytic antibodies
Creators - without role: T.D. Hua - Institut de Génétique Moléculaire de Montpellier
Valérie Rolland - Laboratoire d'aminoacides et peptides
René Lazaro - Laboratoire d'aminoacides et peptides
P. Viallefont - Laboratoire d'aminoacides et peptides
M-P. Lefranc - Université de Montpellier, Institut de Génétique Moléculaire de Montpellier - IGMM
M. Weill - Institut de Génétique Moléculaire de Montpellier
Publication Details: Tetrahedron Letters, Vol.37(2), pp.175-178
Identifiers: 9944806809311
Academic Unit: Institut de Génétique Moléculaire de Montpellier - IGMM; Institut des Biomolécules Max Mousseron - IBMM
Language: English
Resource Type: Journal article
Local Fields: hal-00167764