Database establishment for the secondary fungal DNA barcode translational elongation factor 1α (TEF1α)1

Wieland Meyer; Laszlo Irinyi; Minh Thuy Vi Hoang; Vincent Robert; Dea Garcia-Hermoso; Marie Desnos-Ollivier; Chompoonek Yurayart; Chi-Ching Tsang; Chun-Yi Lee; Patrick C.Y Woo; Ivan Mikhailovich Pchelin; Silke Uhrlaß; Pietro Nenoff; Ariya Chindamporn; Sharon Chen; Paul D.N Hebert; Tania C Sorrell; ISHAM barcoding of pathogenic fungi working group

doi:10.1139/gen-2018-0083

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Database establishment for the secondary fungal DNA barcode translational elongation factor 1α (TEF1α)1

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Database establishment for the secondary fungal DNA barcode translational elongation factor 1α (TEF1α)1

Wieland Meyer, Laszlo Irinyi, Minh Thuy Vi Hoang, Vincent Robert, Dea Garcia-Hermoso, Marie Desnos-Ollivier, Chompoonek Yurayart, Chi-Ching Tsang, Chun-Yi Lee, Patrick C.Y Woo, …

Genome, Vol.62(3), pp.160-169

01/03/2019

DOI: https://doi.org/10.1139/gen-2018-0083

PMID: 30465691

Résumé

base de données d’un code à barres fongique secondaire

codage à barres de l’ADN fongique

facteur d’élongation de la traduction 1α

fungal DNA barcoding

secondary fungal DNA barcode database

translational elongation factor 1α

With new or emerging fungal infections, human and animal fungal pathogens are a growing threat worldwide. Current diagnostic tools are slow, non-specific at the species and subspecies levels, and require specific morphological expertise to accurately identify pathogens from pure cultures. DNA barcodes are easily amplified, universal, short species-specific DNA sequences, which enable rapid identification by comparison with a well-curated reference sequence collection. The primary fungal DNA barcode, ITS region, was introduced in 2012 and is now routinely used in diagnostic laboratories. However, the ITS region only accurately identifies around 75% of all medically relevant fungal species, which has prompted the development of a secondary barcode to increase the resolution power and suitability of DNA barcoding for fungal disease diagnostics. The translational elongation factor 1α (TEF1α) was selected in 2015 as a secondary fungal DNA barcode, but it has not been implemented into practice, due to the absence of a reference database. Here, we have established a quality-controlled reference database for the secondary barcode that together with the ISHAM-ITS database, forms the ISHAM barcode database, available online at http://its.mycologylab.org/ . We encourage the mycology community for active contributions.

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Titre: Database establishment for the secondary fungal DNA barcode translational elongation factor 1α (TEF1α)1
Créateurs - sans rôle: Wieland Meyer - The University of Sydney
Laszlo Irinyi - Molecular Mycology Research Laboratory, Centre for Infectious Diseases and Microbiology, Faculty of Medicine and Health, Sydney School of Medicine, Westmead Clinical School, Marie Bashir Institute for Infectious Diseases and Biosecurity, The University of Sydney, Westmead Hospital (Research and Education Network), Westmead Institute for Medical Research, Westmead, NSW, Australia
Minh Thuy Vi Hoang - Molecular Mycology Research Laboratory, Centre for Infectious Diseases and Microbiology, Faculty of Medicine and Health, Sydney School of Medicine, Westmead Clinical School, Marie Bashir Institute for Infectious Diseases and Biosecurity, The University of Sydney, Westmead Hospital (Research and Education Network), Westmead Institute for Medical Research, Westmead, NSW, Australia
Vincent Robert - Westerdijk Fungal Biodiversity Institute, Utrecht, the Netherlands
Dea Garcia-Hermoso - Institut Pasteur
Marie Desnos-Ollivier - Institut Pasteur, National Reference Center for Invasive Mycoses and Antifungals (NRCMA), Molecular Mycology Unit, CNRS UMR2000, Paris, France
Chompoonek Yurayart - Mycology Unit, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
Chi-Ching Tsang - Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong
Chun-Yi Lee - Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong
Patrick C.Y Woo - University of Hong Kong
Ivan Mikhailovich Pchelin - Laboratory of Molecular Genetic Microbiology, Kashkin Research Institute of Medical Mycology, I.I. Mechnikov North-Western State Medical University, St Petersburg, Russia
Silke Uhrlaß - Laboratory of Medical Microbiology, Partnership Dr. C. Krueger & Prof. Dr. P. Nenoff, Roetha OT Moelbis, Germany
Pietro Nenoff - Laboratory of Medical Microbiology, Partnership Dr. C. Krueger & Prof. Dr. P. Nenoff, Roetha OT Moelbis, Germany
Ariya Chindamporn - Mycology Unit, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
Sharon Chen - Molecular Mycology Research Laboratory, Centre for Infectious Diseases and Microbiology, Faculty of Medicine and Health, Sydney School of Medicine, Westmead Clinical School, Marie Bashir Institute for Infectious Diseases and Biosecurity, The University of Sydney, Westmead Hospital (Research and Education Network), Westmead Institute for Medical Research, Westmead, NSW, Australia
Paul D.N Hebert - Department of Integrative Biology and Director of the Biodiversity Institute of Ontario at the University of Guelph, Guelph, ON, Canada
Tania C Sorrell - Molecular Mycology Research Laboratory, Centre for Infectious Diseases and Microbiology, Faculty of Medicine and Health, Sydney School of Medicine, Westmead Clinical School, Marie Bashir Institute for Infectious Diseases and Biosecurity, The University of Sydney, Westmead Hospital (Research and Education Network), Westmead Institute for Medical Research, Westmead, NSW, Australia
ISHAM barcoding of pathogenic fungi working group
Détails de publication: Genome, Vol.62(3), pp.160-169
Éditeur: NRC Research Press
Nombre de pages: 10
Identifiants: 99159013909311
Unité académique: Maladies Infectieuses et Vecteurs : Ecologie, Génétique, Evolution et Contrôle - MIVEGEC
Langue: English
Type de ressource: Journal article