Résumé
The reniform nematode Rotylenchulus reniformis Linford and Oliveira is becoming one of the most important constraints of cotton production in North and South America. In order to introgress into the main cultivated cotton species Gosypium hirstawn L. the resistance to this parasite from the wild cotton diploid species G. longicalyx, the G. hirsatum x G. thurberi x G. longicalyx trispecific hybrid has been created according to the pseudophyletic method of introgression. The triple species character of the hybrid produced has been proved by using simple sequence repeat (SSR) markers and confirmed by chromosome counting. Three hybrid plants evaluated in controlled conditions of inoculation revealed a high level of resistance to R. reniformis equivalent to that of the diploid donor species.