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Contribution of the classical polymerase chain reaction in the diagnosis of a HIV-1 infected patient in Benin: a case report
Article de revue scientifique   Open Access   Avec comité de lecture

Contribution of the classical polymerase chain reaction in the diagnosis of a HIV-1 infected patient in Benin: a case report

Edmond Tchiakpe, René Keke, Nicole Vidal, Moussa Bachabi, Flore Armande Gangbo, Halimatou Diop-Ndiaye, Coumba Toure-Kane et Akadiri Yessoufou
AIDS Research and Therapy, Vol.18(1)
21/04/2021
PMCID: PMC8059016
PMID: 33882959

Résumé

Benin DNA sequencing Viral load Serology inconclusive HIV-1 Benin / epidemiology HIV Infections* / diagnosis HIV-1* / genetics Humans Polymerase Chain Reaction RNA, Viral Viral Load
Background: First ambitious target by 2020 of UNAIDS is that 90% of people living with HIV know their HIV status. In people older than 18 months of age, serological confirmation test is recommended to confirm HIV infection. Case presentation: Here we report the case of a patient tested positive with HIV-1, ELISA, Murex ® Ag⁄Ab Combination assay (OD450 = 0.802 and cutoff-OD = 0.279) and negative by using FIRST RESPONSE HIV1-2.O CARD TEST (version 2.0) RAPID HIV CARD TEST. Viral load performed with Cobas ® TaqMan ® 96/Cobas ® Ampliprep ® was 6.49log 10. The virus could be sequenced in partial gag and pol genes and belonged to CRF02_AG clade. Conclusion: Conventional PCR is a complementary method for the diagnosis of inconclusive HIV-1 serologies by antibodies.

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