Résumé
A gene fusion system based on plasmid pBBR1MCS and the expression
of green fluorescent protein was developed for Brucella
suis, allowing isolation of constitutive and inducible genes.
Bacteria containing promoter fusions of chromosomal DNA to
gfp were visualized by fluorescence microscopy and examined
by flow cytometry. Twelve clones containing gene fragments induced
inside J774 murine macrophages were isolated and further characterized.