Comparison of the QUANTIPLEX HIV-1 RNA 2.0 Assay with the AMPLICOR HIV-1 MONITOR 1.0 Assay for Quantitation of Levels of Human Immunodeficiency Virus Type 1 RNA in Plasma of Patients Receiving Stavudine-Didanosine Combination Therapy

Michel Segondy; Jacques Izopet; Isabelle Pellegrin; Brigitte Montes; Beatrice Dumon; Christophe Pasquier; Martine Peeters; Herve J. A. Fleury; Jacqueline Puel; Jacques Reynes

doi:10.1128/JCM.36.11.3392-3395.1998

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Comparison of the QUANTIPLEX HIV-1 RNA 2.0 Assay with the AMPLICOR HIV-1 MONITOR 1.0 Assay for Quantitation of Levels of Human Immunodeficiency Virus Type 1 RNA in Plasma of Patients Receiving Stavudine-Didanosine Combination Therapy

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Comparison of the QUANTIPLEX HIV-1 RNA 2.0 Assay with the AMPLICOR HIV-1 MONITOR 1.0 Assay for Quantitation of Levels of Human Immunodeficiency Virus Type 1 RNA in Plasma of Patients Receiving Stavudine-Didanosine Combination Therapy

Michel Segondy, Jacques Izopet, Isabelle Pellegrin, Brigitte Montes, Beatrice Dumon, Christophe Pasquier, Martine Peeters, Herve J. A. Fleury, Jacqueline Puel et Jacques Reynes

Journal of clinical microbiology, Vol.36(11), pp.3392-3395

Note

01/11/1998

DOI: https://doi.org/10.1128/JCM.36.11.3392-3395.1998

PMID: 9774604

Résumé

Virology

We compared the QUANTIPLEX HIV-1 RNA 2.0 assay with the AMPLICOR HIV-1 MONITOR 1.0 assay for quantitation of human immunodeficiency virus type 1 (HIV-1) RNA in plasma in the Stadi trail, which evaluated a stavudine plus didanosine combination therapy in 52 patients. HIV-1 RNA baseline values measured with AMPLICOR HIV-1 MONITOR 1.0 were significantly higher than those measured with QUANTIPLEX HIV-1 RNA 2.0, and decreases in HIV-1 RNA levels from baseline were also found to be significantly higher when measured with the AMPLICOR HIV-1 MONITOR 1.0 assay. The frequency of HIV-1 RNA levels below the lower limit of quantitation was significantly higher with QUANTIPLEX HIV-1 RNA 2.0 than with AMPLICOR HIV-1 MONITOR 1.0. Reanalysis of these results by an ultrasensitive procedure of AMPLICOR HIV-1 MONITOR 1.0 or by a modified version of the test that included additional primers adapted for non-B HIV-1 clades yielded greater differences between the QUANTIPLEX HIV-1 RNA 2.0 assay and the AMPLICOR HIV-1 MONITOR 1.0 assay. Our results indicate that a valid comparison of the virological efficacies obtained with different antiretroviral drug regimens requires the use of the same viral load quantitation procedure; further standardization between the different HIV-1 RNA quantitation kits is therefore needed.

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Titre: Comparison of the QUANTIPLEX HIV-1 RNA 2.0 Assay with the AMPLICOR HIV-1 MONITOR 1.0 Assay for Quantitation of Levels of Human Immunodeficiency Virus Type 1 RNA in Plasma of Patients Receiving Stavudine-Didanosine Combination Therapy
Créateurs - sans rôle: Michel Segondy - Department of Virology and Department of Infectious and Tropical Diseases, Montpellier University Hospital, Montpellier, Department of Virology, Toulouse University Hospital, Toulouse, Department of Virology, Bordeaux University Hospital, Bordeaux, and Retrovirus Laboratory, ORSTOM, Montpellier, France
Jacques Izopet - Department of Virology and Department of Infectious and Tropical Diseases, Montpellier University Hospital, Montpellier, Department of Virology, Toulouse University Hospital, Toulouse, Department of Virology, Bordeaux University Hospital, Bordeaux, and Retrovirus Laboratory, ORSTOM, Montpellier, France
Isabelle Pellegrin - Department of Virology and Department of Infectious and Tropical Diseases, Montpellier University Hospital, Montpellier, Department of Virology, Toulouse University Hospital, Toulouse, Department of Virology, Bordeaux University Hospital, Bordeaux, and Retrovirus Laboratory, ORSTOM, Montpellier, France
Brigitte Montes - Department of Virology and Department of Infectious and Tropical Diseases, Montpellier University Hospital, Montpellier, Department of Virology, Toulouse University Hospital, Toulouse, Department of Virology, Bordeaux University Hospital, Bordeaux, and Retrovirus Laboratory, ORSTOM, Montpellier, France
Beatrice Dumon - Department of Virology and Department of Infectious and Tropical Diseases, Montpellier University Hospital, Montpellier, Department of Virology, Toulouse University Hospital, Toulouse, Department of Virology, Bordeaux University Hospital, Bordeaux, and Retrovirus Laboratory, ORSTOM, Montpellier, France
Christophe Pasquier - Department of Virology and Department of Infectious and Tropical Diseases, Montpellier University Hospital, Montpellier, Department of Virology, Toulouse University Hospital, Toulouse, Department of Virology, Bordeaux University Hospital, Bordeaux, and Retrovirus Laboratory, ORSTOM, Montpellier, France
Martine Peeters - Department of Virology and Department of Infectious and Tropical Diseases, Montpellier University Hospital, Montpellier, Department of Virology, Toulouse University Hospital, Toulouse, Department of Virology, Bordeaux University Hospital, Bordeaux, and Retrovirus Laboratory, ORSTOM, Montpellier, France
Herve J. A. Fleury - Department of Virology and Department of Infectious and Tropical Diseases, Montpellier University Hospital, Montpellier, Department of Virology, Toulouse University Hospital, Toulouse, Department of Virology, Bordeaux University Hospital, Bordeaux, and Retrovirus Laboratory, ORSTOM, Montpellier, France
Jacqueline Puel - Department of Virology and Department of Infectious and Tropical Diseases, Montpellier University Hospital, Montpellier, Department of Virology, Toulouse University Hospital, Toulouse, Department of Virology, Bordeaux University Hospital, Bordeaux, and Retrovirus Laboratory, ORSTOM, Montpellier, France
Jacques Reynes - Department of Virology and Department of Infectious and Tropical Diseases, Montpellier University Hospital, Montpellier, Department of Virology, Toulouse University Hospital, Toulouse, Department of Virology, Bordeaux University Hospital, Bordeaux, and Retrovirus Laboratory, ORSTOM, Montpellier, France
Détails de publication: Journal of clinical microbiology, Vol.36(11), pp.3392-3395
Publications en série: Note
Éditeur: American Society for Microbiology
Nombre de pages: 4
Identifiants: 99158670009311
Unité académique: Recherches translationnelles sur le VIH et les Maladies Infect. Endém. émerg. - TransVIHMI
Langue: English
Type de ressource: Journal article