Comparative induced pluripotent stem cell models of young, aged, and progeroid, as a resource to study human aging trajectories

Human aging is heterogeneous and can be explored through development, physiological aging, and premature accelerated aging. We established human induced pluripotent stem cell (iPSC) models derived from neonatal fibroblasts, peripheral blood mononuclear cells from a healthy 60-year-old donor, and cells from a Hutchinson-Gilford progeria syndrome patient. All lines were generated using Sendai virus reprogramming, validated for pluripotency, tri-lineage differentiation, and genomic stability. This collection provides a unique comparative platform to dissect normal and pathological aging, enabling analyses of youthful resilience, progressive age-related alterations, and premature progeroid hallmarks. Beyond technical validation, these models offer a conceptual framework to identify longevity biomarkers.