Cloning, sequence, and tissue distribution of a rabbit renal Na +/H + exchanger transcript

Friedhelm Hildebrandt; John H. Pizzonia; Robert F. Reilly; Nancy A. Rebouças; Claude Sardet; Jacques Pouysségur; Carolyn W. Slayman; Peter S. Aronson; Peter Igarashi

doi:10.1016/0167-4781(91)90221-7

Retour

Cloning, sequence, and tissue distribution of a rabbit renal Na +/H + exchanger transcript

Article de revue

Avec comité de lecture

Cloning, sequence, and tissue distribution of a rabbit renal Na +/H + exchanger transcript

Friedhelm Hildebrandt, John H. Pizzonia, Robert F. Reilly, Nancy A. Rebouças, Claude Sardet, Jacques Pouysségur, Carolyn W. Slayman, Peter S. Aronson et Peter Igarashi

Biochimica et biophysica acta. Gene structure and expression, Vol.1129(1), pp.105-108

02/12/1991

DOI: https://doi.org/10.1016/0167-4781(91)90221-7

PMID: 1661611

Résumé

Amino acid sequence

cDNA cloning

Polymerase chain reaction

Rabbit kidney

Sodium-proton exchange

Rabbit kidney expresses a transcript that is similar to the human growth-factor-activatable Na +/H + exchanger. PCR and library screening were used to clone overlapping 2.5 kb, 1.4 kb, and 1.8 kb cDNAs that together contain the entire coding region (2448 bp) and 5′ untranslated region (726 bp) and part of the 3′ untranslated region (128 bp) of a rabbit renal Na +/H + exchanger transcript. The nucleotide and inferred amino acid sequences are highly conserved between rabbit and human (88% nucleotide identity, 95% amino acid identity). In rabbit, the transcript is expressed in both epithelial and non-epithelial tissues, with highes expression in stomach, brain, kidney, lung and ileum, and minimal expression in liver and skeletal muscle.

Indicateurs

1 Consultations de la notice

Détails

Titre: Cloning, sequence, and tissue distribution of a rabbit renal Na +/H + exchanger transcript
Créateurs - sans rôle: Friedhelm Hildebrandt - Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, U.S.A
John H. Pizzonia - Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, U.S.A
Robert F. Reilly - Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, U.S.A
Nancy A. Rebouças - Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, U.S.A
Claude Sardet - Centre de Biochimie-CNRS, Université de Nice, Nice, France
Jacques Pouysségur - Centre de Biochimie-CNRS, Université de Nice, Nice, France
Carolyn W. Slayman - Department of Human Genetics, Yale University School of Medicine, New Haven, CT, U.S.A
Peter S. Aronson - Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, U.S.A
Peter Igarashi - Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, U.S.A
Détails de publication: Biochimica et biophysica acta. Gene structure and expression, Vol.1129(1), pp.105-108
Éditeur: Elsevier B.V
Nombre de pages: 4
Identifiants: 99162650309311
Unité académique: Institut de Recherche en Cancérologie de Montpellier - IRCM
Langue: English
Type de ressource: Journal article