Cloning of a cDNA encoding an E2 ubiquitin‐conjugating enzyme from Catharanthus roseus: expression analysis in plant organs and in response to hormones in cell suspensions

Yann Sibéril; Martine Thiersault; Gonçalo Nepumoceno; Pierre Doireau; Pascal Gantet

doi:10.1093/jexbot/53.366.149

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Cloning of a cDNA encoding an E2 ubiquitin‐conjugating enzyme from Catharanthus roseus: expression analysis in plant organs and in response to hormones in cell suspensions

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Cloning of a cDNA encoding an E2 ubiquitin‐conjugating enzyme from Catharanthus roseus: expression analysis in plant organs and in response to hormones in cell suspensions

Yann Sibéril, Martine Thiersault, Gonçalo Nepumoceno, Pierre Doireau et Pascal Gantet

Journal of experimental botany, Vol.53(366), pp.149-150

01/01/2002

DOI: https://doi.org/10.1093/jexbot/53.366.149

PMID: 11741052

Résumé

Catharanthus roseus

cytokinin

E2 ubiquitin‐conjugating enzyme

A novel cDNA (Crubie2) encoding ubiquitin‐conjugating enzyme E2 was isolated from a Catharanthus roseus cDNA library. Sequence comparison with Arabidopsis thaliana E2 sequences revealed that CrUBIE2 is a member of a new plant E2 sub‐family. Expression of Crubie2 is repressed in developing organs and down‐regulated by cytokinin suggesting that a decrease in the ubiquitin‐dependent proteolytic pathway may take part in the regulation of alkaloid biosynthesis in C. roseus cell suspensions.

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Titre: Cloning of a cDNA encoding an E2 ubiquitin‐conjugating enzyme from Catharanthus roseus: expression analysis in plant organs and in response to hormones in cell suspensions
Créateurs - sans rôle: Yann Sibéril - EA 2106, Biomolécules et Biotechnologies Végétales, Université de Tours, UFR des Sciences et Techniques, Laboratoire de Physiologie Végétale, Parc de Grandmont, 37200 Tours, France
Martine Thiersault - EA 2106, Biomolécules et Biotechnologies Végétales, Université de Tours, UFR des Sciences et Techniques, Laboratoire de Physiologie Végétale, Parc de Grandmont, 37200 Tours, France
Gonçalo Nepumoceno - EA 2106, Biomolécules et Biotechnologies Végétales, Université de Tours, UFR des Sciences et Techniques, Laboratoire de Physiologie Végétale, Parc de Grandmont, 37200 Tours, France
Pierre Doireau - Université de Tours
Pascal Gantet - Université de Tours
Détails de publication: Journal of experimental botany, Vol.53(366), pp.149-150
Éditeur: Oxford University Press
Nombre de pages: 2
Identifiants: 99136459609311
Unité académique: Diversité, Adaptation et Développement des plantes - DIADE
Langue: English
Type de ressource: Journal article