Résumé
To investigate the molecular basis of the human
SRY gene regulation, we have examined the significance of two potential binding sites for the transcription factor Sp1 (Sp1A: −124 to −131 and Sp1B: −147 to −154) by DNase I footprinting and gel mobility shift assays. Cotransfection experiments in
Drosophila SL2 cells implicated Sp1 protein in the transcriptional activation of the SRY promoter.