Abstract
Premise of the study: Multiplexed microsatellite markers were developed for population genetic studies in the pseudometallophyteNoccaea caerulescens (Brassicaceae), a model species to investigate metal tolerance and hyperaccumulation in higherplants.• Methods and Results: Microsatellite loci were isolated through pyrosequencing of an enriched DNA library. Three multiplexescombining four previously published and 17 newly designed markers were developed. The new markers were screened inmetallicolous and nonmetallicolous populations from southern France. The total number of alleles per locus ranged from fi veto 18. The observed heterozygosity per locus and per population ranged from 0 to 0.83, and expected heterozygosity rangedfrom 0 to 0.89.• Conclusions: The investigated loci showed reasonable to high levels of polymorphism at the regional scale. The multiplex setshould be helpful in investigating genetic diversity, population structure, and demographic history in N. caerulescens at variousspatial scales.