Broad-spectrum resistance to bacterial blight in rice using genome editing

Ricardo Oliva; Chonghui Ji; Genelou Atienza-Grande; José C Huguet-Tapia; Alvaro Perez-Quintero; Ting Li; Joon-Seob Eom; Chenhao Li; Hanna Nguyen; Bo Liu; Florence Auguy; Coline Sciallano; Van T Luu; Gerbert S Dossa; Sébastien Cunnac; Sarah M Schmidt; Inez H Slamet-Loedin; Casiana Vera Cruz; Boris Szurek; Wolf B Frommer; Frank F White; Bing Yang

doi:10.1038/s41587-019-0267-z

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Broad-spectrum resistance to bacterial blight in rice using genome editing

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Broad-spectrum resistance to bacterial blight in rice using genome editing

Ricardo Oliva, Chonghui Ji, Genelou Atienza-Grande, José C Huguet-Tapia, Alvaro Perez-Quintero, Ting Li, Joon-Seob Eom, Chenhao Li, Hanna Nguyen, Bo Liu, …

Nature biotechnology, Vol.37(11), pp.1344-1350

01/11/2019

DOI: https://doi.org/10.1038/s41587-019-0267-z

PMID: 31659337

Résumé

Bacterial Proteins - genetics

CRISPR-Cas Systems

Disease Resistance

Gene Editing

Gene Expression Regulation, Plant

Membrane Transport Proteins - genetics

Mutation

Oryza - genetics

Oryza - growth & development

Oryza - microbiology

Plant Proteins - genetics

Promoter Regions, Genetic

Sequence Analysis, DNA

Transcription Activator-Like Effectors - genetics

Xanthomonas - genetics

Xanthomonas - pathogenicity

Bacterial blight of rice is an important disease in Asia and Africa. The pathogen, Xanthomonas oryzae pv. oryzae (Xoo), secretes one or more of six known transcription-activator-like effectors (TALes) that bind specific promoter sequences and induce, at minimum, one of the three host sucrose transporter genes SWEET11, SWEET13 and SWEET14, the expression of which is required for disease susceptibility. We used CRISPR-Cas9-mediated genome editing to introduce mutations in all three SWEET gene promoters. Editing was further informed by sequence analyses of TALe genes in 63 Xoo strains, which revealed multiple TALe variants for SWEET13 alleles. Mutations were also created in SWEET14, which is also targeted by two TALes from an African Xoo lineage. A total of five promoter mutations were simultaneously introduced into the rice line Kitaake and the elite mega varieties IR64 and Ciherang-Sub1. Paddy trials showed that genome-edited SWEET promoters endow rice lines with robust, broad-spectrum resistance.

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Titre: Broad-spectrum resistance to bacterial blight in rice using genome editing
Créateurs - sans rôle: Ricardo Oliva - International Rice Research Institute
Chonghui Ji - University of Missouri
Genelou Atienza-Grande - College of Agriculture and Food Science, University of the Philippines Los Baños, Los Baños, Philippines
José C Huguet-Tapia - University of Florida
Alvaro Perez-Quintero - Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO, USA
Ting Li - Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, USA
Joon-Seob Eom - Institute for Molecular Physiology and Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich Heine Universität Düsseldorf and Max Planck Institute for Plant Breeding Research, Köln, Germany
Chenhao Li - Division of Plant Sciences, Bond Life Sciences Center, University of Missouri, Columbia, MO, USA
Hanna Nguyen - International Rice Research Institute, Metro Manila, Philippines
Bo Liu - Division of Plant Sciences, Bond Life Sciences Center, University of Missouri, Columbia, MO, USA
Florence Auguy - IRD, CIRAD, Université Montpellier, IPME, Montpellier, France
Coline Sciallano - IRD, CIRAD, Université Montpellier, IPME, Montpellier, France
Van T Luu - Institute for Molecular Physiology and Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich Heine Universität Düsseldorf and Max Planck Institute for Plant Breeding Research, Köln, Germany
Gerbert S Dossa - Erfurt University of Applied Sciences, Erfurt, Germany
Sébastien Cunnac - IRD, CIRAD, Université Montpellier, IPME, Montpellier, France
Sarah M Schmidt - Institute for Molecular Physiology and Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich Heine Universität Düsseldorf and Max Planck Institute for Plant Breeding Research, Köln, Germany
Inez H Slamet-Loedin - International Rice Research Institute, Metro Manila, Philippines
Casiana Vera Cruz - International Rice Research Institute, Metro Manila, Philippines
Boris Szurek - IRD, CIRAD, Université Montpellier, IPME, Montpellier, France
Wolf B Frommer - Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Aichi, Japan. frommew@hhu.de
Frank F White - University of Florida
Bing Yang - Donald Danforth Plant Science Center, St. Louis, MO, USA. yangbi@missouri.edu
Détails de publication: Nature biotechnology, Vol.37(11), pp.1344-1350
Identifiants: 99157274309311
Unité académique: Plant Health Institute of Montpellier - PHIM
Langue: English
Type de ressource: Journal article