AvrAC Xcc8004 , a Type III Effector with a Leucine-Rich Repeat Domain from Xanthomonas campestris Pathovar campestris Confers Avirulence in Vascular Tissues of Arabidopsis thaliana Ecotype Col-0

Rong-Qi Xu; Servane Blanvillain; Jia-Xun Feng; Bo-Le Jiang; Xian-Zhen Li; Hong-Yu Wei; Thomas Kroj; Emmanuelle Lauber; Dominique Roby; Baoshan Chen; Yong-Qiang He; Guang-Tao Lu; Dong-Jie Tang; Jacques Vasse; Matthieu Arlat; Ji-Liang Tang

doi:10.1128/JB.00978-07

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AvrAC Xcc8004 , a Type III Effector with a Leucine-Rich Repeat Domain from Xanthomonas campestris Pathovar campestris Confers Avirulence in Vascular Tissues of Arabidopsis thaliana Ecotype Col-0

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AvrAC Xcc8004 , a Type III Effector with a Leucine-Rich Repeat Domain from Xanthomonas campestris Pathovar campestris Confers Avirulence in Vascular Tissues of Arabidopsis thaliana Ecotype Col-0

Rong-Qi Xu, Servane Blanvillain, Jia-Xun Feng, Bo-Le Jiang, Xian-Zhen Li, Hong-Yu Wei, Thomas Kroj, Emmanuelle Lauber, Dominique Roby, Baoshan Chen, …

Journal of bacteriology, Vol.190(1), pp.343-355

01/2008

DOI: https://doi.org/10.1128/JB.00978-07

Résumé

Xanthomonas campestris pathovar campestris causes black rot, a vascular disease on cruciferous plants, including Arabidopsis thaliana . The gene XC1553 from X. campestris pv. campestris strain 8004 encodes a protein containing leucine-rich repeats (LRRs) and appears to be restricted to strains of X. campestris pv. campestris. LRRs are found in a number of type III-secreted effectors in plant and animal pathogens. These prompted us to investigate the role of the XC1553 gene in the interaction between X. campestris pv. campestris and A. thaliana . Translocation assays using the hypersensitive-reaction-inducing domain of X. campestris pv. campestris AvrBs1 as a reporter revealed that XC1553 is a type III effector. Infiltration of Arabidopsis leaf mesophyll with bacterial suspensions showed no differences between the wild-type strain and an XC1553 gene mutant; both strains induced disease symptoms on Kashmir and Col-0 ecotypes. However, a clear difference was observed when bacteria were introduced into the vascular system by piercing the central vein of leaves. In this case, the wild-type strain 8004 caused disease on the Kashmir ecotype, but not on ecotype Col-0; the XC1553 gene mutant became virulent on the Col-0 ecotype and still induced disease on the Kashmir ecotype. Altogether, these data show that the XC1553 gene, which was renamed avrAC Xcc8004 , functions as an avirulence gene whose product seems to be recognized in vascular tissues.

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Titre: AvrAC Xcc8004 , a Type III Effector with a Leucine-Rich Repeat Domain from Xanthomonas campestris Pathovar campestris Confers Avirulence in Vascular Tissues of Arabidopsis thaliana Ecotype Col-0
Créateurs - sans rôle: Rong-Qi Xu - Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, The Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, and College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China
Servane Blanvillain - Laboratoire des Interactions Plantes-Microorganismes, INRA-CNRS, BP52627, 31326 Castanet Cedex, France
Jia-Xun Feng - Guangxi University
Bo-Le Jiang - Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, The Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, and College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China
Xian-Zhen Li - Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, The Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, and College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China
Hong-Yu Wei - Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, The Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, and College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China
Thomas Kroj - Laboratoire des Interactions Plantes-Microorganismes, INRA-CNRS, BP52627, 31326 Castanet Cedex, France
Emmanuelle Lauber - Laboratoire des Interactions Plantes-Microorganismes, INRA-CNRS, BP52627, 31326 Castanet Cedex, France
Dominique Roby - Laboratoire des Interactions Plantes-Microorganismes, INRA-CNRS, BP52627, 31326 Castanet Cedex, France
Baoshan Chen - Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, The Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, and College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China
Yong-Qiang He - Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, The Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, and College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China
Guang-Tao Lu - Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, The Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, and College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China
Dong-Jie Tang - Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, The Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, and College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China
Jacques Vasse - Laboratoire des Interactions Plantes-Microorganismes, INRA-CNRS, BP52627, 31326 Castanet Cedex, France
Matthieu Arlat - Laboratoire des Interactions Plantes-Microorganismes, INRA-CNRS, BP52627, 31326 Castanet Cedex, France, Université Paul Sabatier, Toulouse III, Toulouse, France
Ji-Liang Tang - Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, The Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, and College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, People's Republic of China
Détails de publication: Journal of bacteriology, Vol.190(1), pp.343-355
Identifiants: 99157175109311
Unité académique: Plant Health Institute of Montpellier - PHIM
Langue: English
Type de ressource: Journal article