An international standardization programme towards the application of gene expression profiling in routine leukaemia diagnostics: the Microarray Innovations in LEukemia study prephase

Alexander Kohlmann; Thomas J Kipps; Laura Z Rassenti; James R Downing; Sheila A Shurtleff; Ken I Mills; Amanda F Gilkes; Wolf-Karsten Hofmann; Giuseppe Basso; Marta Campo Dell’Orto; Robin Foà; Sabina Chiaretti; John De Vos; Sonja Rauhut; Peter R Papenhausen; Jesus M Hernández; Eva Lumbreras; Allen E Yeoh; Evelyn S Koay; Rachel Li; Wei-min Liu; Paul M Williams; Lothar Wieczorek; Torsten Haferlach

doi:10.1111/j.1365-2141.2008.07261.x

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An international standardization programme towards the application of gene expression profiling in routine leukaemia diagnostics: the Microarray Innovations in LEukemia study prephase

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An international standardization programme towards the application of gene expression profiling in routine leukaemia diagnostics: the Microarray Innovations in LEukemia study prephase

Alexander Kohlmann, Thomas J Kipps, Laura Z Rassenti, James R Downing, Sheila A Shurtleff, Ken I Mills, Amanda F Gilkes, Wolf-Karsten Hofmann, Giuseppe Basso, Marta Campo Dell’Orto, …

British journal of haematology, Vol.142(5), pp.802-807

09/2008

DOI: https://doi.org/10.1111/j.1365-2141.2008.07261.x

PMID: 18573112

Résumé

Haematological Malignancy

Gene expression profiling has the potential to enhance current methods for the diagnosis of haematological malignancies. Here, we present data on 204 analyses from an international standardization programme that was conducted in 11 laboratories as a prephase to the Microarray Innovations in LEukemia (MILE) study. Each laboratory prepared two cell line samples, together with three replicate leukaemia patient lysates in two distinct stages: (i) a 5-d course of protocol training, and (ii) independent proficiency testing. Unsupervised, supervised, and r2 correlation analyses demonstrated that microarray analysis can be performed with remarkably high intra-laboratory reproducibility and with comparable quality and reliability.

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Titre: An international standardization programme towards the application of gene expression profiling in routine leukaemia diagnostics: the Microarray Innovations in LEukemia study prephase
Créateurs - sans rôle: Alexander Kohlmann - Roche Molecular Systems, Inc., Department of Genomics and Oncology
Thomas J Kipps - Moores Cancer Center
Laura Z Rassenti - Moores Cancer Center, University of California
James R Downing - Department of Pathology, St Jude Children's Research Hospital
Sheila A Shurtleff - Department of Pathology, St Jude Children's Research Hospital
Ken I Mills - Department of Haematology, University of Cardiff
Amanda F Gilkes - Department of Haematology, University of Cardiff
Wolf-Karsten Hofmann - Charité, University Hospital Benjamin Franklin
Giuseppe Basso - Department of Paediatrics, University of Padua
Marta Campo Dell’Orto - Department of Paediatrics, University of Padua
Robin Foà - Division of Haematology, Department of Cellular Biotechnologies and Haematology, University “La Sapienza”
Sabina Chiaretti - Division of Haematology, Department of Cellular Biotechnologies and Haematology, University “La Sapienza”
John De Vos - Institut de Recherche en Biothérapie, CHU de Montpellier
Sonja Rauhut - Munich Leukemia Laboratory
Peter R Papenhausen - Laboratory Corporation of America, RTP, NC
Jesus M Hernández - Hospital Universitario de Salamanca and Centro de Investigación del Cáncer (CIC), Universidad de Salamanca-CSIC
Eva Lumbreras - Hospital Universitario de Salamanca and Centro de Investigación del Cáncer (CIC), Universidad de Salamanca-CSIC
Allen E Yeoh - Departments of Pathology and Paediatrics, National University of Singapore
Evelyn S Koay - Departments of Pathology and Paediatrics, National University of Singapore
Rachel Li - Roche Molecular Systems, Inc., Department of Genomics and Oncology
Wei-min Liu - Roche Molecular Systems, Inc., Department of Genomics and Oncology
Paul M Williams - Roche Molecular Systems, Inc., Department of Genomics and Oncology
Lothar Wieczorek - Roche Molecular Systems, Inc., Department of Genomics and Oncology
Torsten Haferlach - Munich Leukemia Laboratory
Détails de publication: British journal of haematology, Vol.142(5), pp.802-807
Éditeur: Blackwell Publishing Ltd
Nombre de pages: 6
Identifiants: 99141206309311
Unité académique: Cell souches plasticité cellul régénérat tissulaire immuno des maladies inflamm - IRMB
Langue: English
Type de ressource: Journal article