An RNA-centric dissection of host complexes controlling flavivirus infection

Yaw Shin Ooi; Karim Majzoub; Ryan A Flynn; Miguel A Mata; Jonathan Diep; Jason Kenichi Li; Nicholas van Buuren; Neil Rumachik; Alex G Johnson; Andreas S Puschnik; Caleb D Marceau; Luwanika Mlera; Jeffrey M Grabowski; Karla Kirkegaard; Marshall E Bloom; Peter Sarnow; Carolyn R Bertozzi; Jan E Carette

doi:10.1038/s41564-019-0518-2

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An RNA-centric dissection of host complexes controlling flavivirus infection

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An RNA-centric dissection of host complexes controlling flavivirus infection

Yaw Shin Ooi, Karim Majzoub, Ryan A Flynn, Miguel A Mata, Jonathan Diep, Jason Kenichi Li, Nicholas van Buuren, Neil Rumachik, Alex G Johnson, Andreas S Puschnik, …

Nature microbiology, Vol.4(12), pp.2369-2382

01/12/2019

DOI: https://doi.org/10.1038/s41564-019-0518-2

PMID: 31384002

Résumé

Carrier Proteins

Cell Line

CRISPR-Cas Systems

Dengue Virus - genetics

Endoplasmic Reticulum - genetics

Endoplasmic Reticulum - metabolism

Flavivirus - genetics

Flavivirus - pathogenicity

Flavivirus - physiology

Flavivirus Infections - virology

Gene Knockout Techniques

Host-Pathogen Interactions - genetics

Humans

RNA, Viral - genetics

RNA, Viral - metabolism

RNA-Binding Proteins - genetics

Virus Replication

Zika Virus - genetics

Flaviviruses, including dengue virus (DENV) and Zika virus (ZIKV), cause severe human disease. Co-opting cellular factors for viral translation and viral genome replication at the endoplasmic reticulum is a shared replication strategy, despite different clinical outcomes. Although the protein products of these viruses have been studied in depth, how the RNA genomes operate inside human cells is poorly understood. Using comprehensive identification of RNA-binding proteins by mass spectrometry (ChIRP-MS), we took an RNA-centric viewpoint of flaviviral infection and identified several hundred proteins associated with both DENV and ZIKV genomic RNA in human cells. Genome-scale knockout screens assigned putative functional relevance to the RNA-protein interactions observed by ChIRP-MS. The endoplasmic-reticulum-localized RNA-binding proteins vigilin and ribosome-binding protein 1 directly bound viral RNA and each acted at distinct stages in the life cycle of flaviviruses. Thus, this versatile strategy can elucidate features of human biology that control the pathogenesis of clinically relevant viruses.

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Titre: An RNA-centric dissection of host complexes controlling flavivirus infection
Créateurs - sans rôle: Yaw Shin Ooi - Department of Microbiology and Immunology, Stanford University, Stanford, CA, USA
Karim Majzoub - Inserm
Ryan A Flynn - Department of Chemistry, Stanford University, Stanford, CA, USA. raflynn@stanford.edu
Miguel A Mata - Department of Microbiology and Immunology, Stanford University, Stanford, CA, USA
Jonathan Diep - Department of Microbiology and Immunology, Stanford University, Stanford, CA, USA
Jason Kenichi Li - Department of Genetics, Stanford University, Stanford, CA, USA
Nicholas van Buuren - Department of Genetics, Stanford University, Stanford, CA, USA
Neil Rumachik - Department of Chemistry, Stanford University, Stanford, CA, USA
Alex G Johnson - Department of Chemical and Systems Biology, Stanford University, Stanford, CA, USA
Andreas S Puschnik - Department of Microbiology and Immunology, Stanford University, Stanford, CA, USA
Caleb D Marceau - Department of Microbiology and Immunology, Stanford University, Stanford, CA, USA
Luwanika Mlera - Biology of Vector-Borne Viruses Section, Rocky Mountain Laboratories, NIAID/NIH, Hamilton, MT, USA
Jeffrey M Grabowski - Biology of Vector-Borne Viruses Section, Rocky Mountain Laboratories, NIAID/NIH, Hamilton, MT, USA
Karla Kirkegaard - Department of Genetics, Stanford University, Stanford, CA, USA
Marshall E Bloom - Biology of Vector-Borne Viruses Section, Rocky Mountain Laboratories, NIAID/NIH, Hamilton, MT, USA
Peter Sarnow - Department of Microbiology and Immunology, Stanford University, Stanford, CA, USA
Carolyn R Bertozzi - Stanford University
Jan E Carette - Stanford University
Détails de publication: Nature microbiology, Vol.4(12), pp.2369-2382
Note de subvention: R01 AI134912 / NIAID NIH HHS R01 AI047365 / NIAID NIH HHS R01 AI141970 / NIAID NIH HHS R01 AI069000 / NIAID NIH HHS U19 AI109662 / NIAID NIH HHS DP2 AI104557 / NIAID NIH HHS R01 AI051622 / NIAID NIH HHS R37 AI047365 / NIAID NIH HHS R37 AI051622 / NIAID NIH HHS
Identifiants: 99153927409311
Unité académique: Institut de Génétique Moléculaire de Montpellier - IGMM
Langue: English
Type de ressource: Journal article