Amphipols From A to Z

Jean-Luc Popot; Thorsten Althoff; Dominique Bagnard; Jean-Louis Banères; Paola Bazzacco; Emmanuelle Billon-Denis; Laurent J Catoire; Philippe Champeil; Delphine Charvolin; Melanie J. Cocco; Gérard Cremel; Tassadite Dahmane; Luis M. de La Maza; Christine Ebel; Frank Gabel; Francesco Giusti; Yann Gohon; Erik Goormaghtigh; Eric Guittet; Jörg H. Kleinschmidt; Werner Kühlbrandt; Christel Le Bon; Karen L. Martinez; Martin Picard; Bernard Pucci; Jonathan Sachs; Christophe Tribet; Carine van Heijenoort; Frank Wien; Francesca Zito; Manuela Zoonens

doi:10.1146/annurev-biophys-042910-155219

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Amphipols From A to Z

Jean-Luc Popot, Thorsten Althoff, Dominique Bagnard, Jean-Louis Banères, Paola Bazzacco, Emmanuelle Billon-Denis, Laurent J Catoire, Philippe Champeil, Delphine Charvolin, Melanie J. Cocco, …

Annual Review of Biophysics and Biomolecular Structure, Vol.40(1), pp.379-408

09/06/2011

DOI: https://doi.org/10.1146/annurev-biophys-042910-155219

Abstract

Amphipathic polymers

Membrane biochemistry

Membrane proteins

Membrane biophysics

In-vitro

Transmembrane Domain

Aqueous solutions

Coupled receptors

Amphiphilic polymers

Amphipols (APols) are short amphipathic polymers that can substitute for detergents to keep integral membrane proteins (MPs) water soluble. In this review, we discuss their structure and solution behavior; the way they associate with MPs; and the structure, dynamics, and solution properties of the resulting complexes. All MPs tested to date form water-soluble complexes with APols, and their biochemical stability is in general greatly improved compared with MPs in detergent solutions. The functionality and ligand-binding properties of APol-trapped MPs are reviewed, and the mechanisms by which APols stabilize MPs are discussed. Applications of APols include MP folding and cell-free synthesis, structural studies by NMR, electron microscopy and X-ray diffraction, APol-mediated immobilization of MPs onto solid supports, proteomics, delivery of MPs to preexisting membranes, and vaccine formulation.

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Title: Amphipols From A to Z
Creators - without role: Jean-Luc Popot - Institut de Biologie Physico-Chimique
Thorsten Althoff - Centre National de la Recherche Scientifique
Dominique Bagnard - Centre National de la Recherche Scientifique
Jean-Louis Banères - Université de Montpellier, Institut des Biomolécules Max Mousseron - IBMM
Paola Bazzacco - Institut de Biologie Physico-Chimique
Emmanuelle Billon-Denis - Centre National de la Recherche Scientifique
Laurent J Catoire - Centre National de la Recherche Scientifique
Philippe Champeil - Centre National de la Recherche Scientifique
Delphine Charvolin - Centre National de la Recherche Scientifique
Melanie J. Cocco - Centre National de la Recherche Scientifique
Gérard Cremel - Centre National de la Recherche Scientifique
Tassadite Dahmane - Centre National de la Recherche Scientifique
Luis M. de La Maza - Centre National de la Recherche Scientifique
Christine Ebel - Institut de Biologie Structurale
Frank Gabel - Institut de Biologie Structurale
Francesco Giusti - Laboratoire de Biologie Physico-Chimique des Protéines Membranaires
Yann Gohon - Institut de Biologie Physico-Chimique
Erik Goormaghtigh - Centre National de la Recherche Scientifique
Eric Guittet - Institut de Chimie des Substances Naturelles
Jörg H. Kleinschmidt - Centre National de la Recherche Scientifique
Werner Kühlbrandt - Centre National de la Recherche Scientifique
Christel Le Bon - Laboratoire de Biologie Physico-Chimique des Protéines Membranaires
Karen L. Martinez - Centre National de la Recherche Scientifique
Martin Picard - Centre National de la Recherche Scientifique
Bernard Pucci - Institut de Biologie Physico-Chimique
Jonathan Sachs - Centre National de la Recherche Scientifique
Christophe Tribet - Centre National de la Recherche Scientifique
Carine van Heijenoort - Institut de Chimie des Substances Naturelles
Frank Wien - Centre National de la Recherche Scientifique
Francesca Zito - Institut de Biologie Physico-Chimique
Manuela Zoonens - Centre National de la Recherche Scientifique
Publication Details: Annual Review of Biophysics and Biomolecular Structure, Vol.40(1), pp.379-408
Identifiers: 9935463709311
Academic Unit: Institut des Biomolécules Max Mousseron - IBMM
Language: English
Resource Type: Journal article
Local Fields: hal-02327349