Résumé
P2X sub(4) purinergic receptors are calcium-permeable, ATP-activated ion channels. In the CA1 area of the hippocampus, they are located at the subsynaptic membrane somewhat peripherally to AMPA receptors. The possible role of P2X sub(4) receptors has been difficult to elucidate because of the lack of selective antagonists. Here we report the generation of a P2X sub(4) receptor knock-out mouse and show that long-term potentiation (LTP) at Schaffer collateral synapses is reduced relative to that in wild-type mice. Ivermectin, which selectively potentiates currents at P2X sub(4), was found to increase LTP in wild-type mice but had no effect in P2X sub(4) knock-out mice. We suggest that calcium entry through subsynaptic P2X sub(4) receptors during high-frequency stimulation contributes to synaptic strengthening.