Activation of c- myb by 5′ retrovirus promoter insertion in myeloid neoplasms is dependent upon an intact alternative splice donor site (SD′) in gag

Jean Marie Ramirez; Laurent Houzet; Richard Koller; Juraj Bies; Linda Wolff; Marylène Mougel

doi:10.1016/j.virol.2004.09.038

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Activation of c- myb by 5′ retrovirus promoter insertion in myeloid neoplasms is dependent upon an intact alternative splice donor site (SD′) in gag

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Activation of c- myb by 5′ retrovirus promoter insertion in myeloid neoplasms is dependent upon an intact alternative splice donor site (SD′) in gag

Jean Marie Ramirez, Laurent Houzet, Richard Koller, Juraj Bies, Linda Wolff et Marylène Mougel

Virology (New York, N.Y.), Vol.330(2), pp.398-407

20/12/2004

DOI: https://doi.org/10.1016/j.virol.2004.09.038

PMID: 15567434

Résumé

c- myb

Leukemia

Lymphoma

MuLV

Myeloid neoplasm

Oncogene

Splicing

Tumorigenesis

Alternative splicing in Mo-MuLV recruits a splice donor site, SD′, within the gag that is required for optimal replication in vitro. Remarkably, this SD′ site was also found to be utilized for production of oncogenic gag-myb fusion RNA in 100% of murine-induced myeloid leukemia (MML) in pristane-treated BALB/c mice. Therefore, we investigated the influence of silent mutations of SD′ in this model. Although there was no decrease in the overall incidence of disease, there was a decrease in the incidence of myeloid leukemia with a concomitant increase in lymphoid leukemia. Importantly, there was a complete lack of myeloid tumors associated with 5′ insertional mutagenic activation of c- myb, suggesting the specific requirement of the SD′ site in this mechanism.

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Détails

Titre: Activation of c- myb by 5′ retrovirus promoter insertion in myeloid neoplasms is dependent upon an intact alternative splice donor site (SD′) in gag
Créateurs - sans rôle: Jean Marie Ramirez - Laboratoire Infections Rétrovirales et Signalisation Cellulaire, CNRS UMR5121, UMI, IFR122, Montpellier, France
Laurent Houzet - Laboratoire Infections Rétrovirales et Signalisation Cellulaire, CNRS UMR5121, UMI, IFR122, Montpellier, France
Richard Koller - Laboratory of Cellular Oncology, National Cancer Institute, NIH, Bethesda, MD 20892-4263, USA
Juraj Bies - Laboratory of Cellular Oncology, National Cancer Institute, NIH, Bethesda, MD 20892-4263, USA
Linda Wolff - Laboratory of Cellular Oncology, National Cancer Institute, NIH, Bethesda, MD 20892-4263, USA
Marylène Mougel - Laboratoire Infections Rétrovirales et Signalisation Cellulaire, CNRS UMR5121, UMI, IFR122, Montpellier, France
Détails de publication: Virology (New York, N.Y.), Vol.330(2), pp.398-407
Éditeur: Elsevier Inc
Nombre de pages: 10
Identifiants: 99155339009311
Unité académique: Institut des Biomolécules Max Mousseron - IBMM; Institut de Recherche en Infectiologie de Montpellier - IRIM
Langue: English
Type de ressource: Journal article