A reversible gene trap collection empowers haploid genetics in human cells

Tilmann Buerckstuemmer; Carina Banning; Philipp Hainzl; Richard Schobesberger; Claudia Kerzendorfer; Florian M. Pauler; Doris Chen; Nicole Them; Fiorella Schischlik; Manuele Rebsamen; Michal Smida; Ferran Fece de la Cruz; Ana Lapao; Melissa Liszt; Benjamin Eizinger; Philipp M. Guenzl; Vincent A. Blomen; Tomasz Konopka; Bianca Gapp; Katja Parapatics; Barbara Maier; Johannes Stoeckl; Wolfgang Fischl; Sejla Salic; M. Rita Taba Casari; Sylvia Knapp; Keiryn L. Bennett; Christoph Bock; Jacques Colinge; Robert Kralovics; Gustav Ammerer; Georg Casari; Thijn R. Brummelkamp; Giulio Superti-Furga; Sebastian M. B. Nijman

doi:10.1038/nmeth.2609

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A reversible gene trap collection empowers haploid genetics in human cells

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A reversible gene trap collection empowers haploid genetics in human cells

Tilmann Buerckstuemmer, Carina Banning, Philipp Hainzl, Richard Schobesberger, Claudia Kerzendorfer, Florian M. Pauler, Doris Chen, Nicole Them, Fiorella Schischlik, Manuele Rebsamen, …

Nature methods, Vol.10(10), pp.965-971

01/10/2013

DOI: https://doi.org/10.1038/nmeth.2609

PMID: 24161985

Résumé

Biochemical Research Methods

Biochemistry & Molecular Biology

Life Sciences & Biomedicine

Science & Technology

Knockout collections are invaluable tools for studying model organisms such as yeast. However, there are no largescale knockout collections of human cells. Using gene-trap mutagenesis in near-haploid human cells, we established a platform to generate and isolate individual 'gene-trapped cells' and used it to prepare a collection of human cell lines carrying single gene-trap insertions. In most cases, the insertion can be reversed. This growing library covers 3,396 genes, one-third of the expressed genome, is DNA-barcoded and allows systematic screens for a wide variety of cellular phenotypes. We examined cellular responses to TNF-alpha, TGF-beta, IFN-gamma and TNF-related apoptosis-inducing ligand (TRAIL), to illustrate the value of this unique collection of isogenic human cell lines.

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Titre: A reversible gene trap collection empowers haploid genetics in human cells
Créateurs - sans rôle: Tilmann Buerckstuemmer - Haplogen GmbH, Vienna, Austria
Carina Banning - Haplogen GmbH, Vienna, Austria
Philipp Hainzl - Haplogen GmbH, Vienna, Austria
Richard Schobesberger - Haplogen GmbH, Vienna, Austria
Claudia Kerzendorfer - CeMM Research Center for Molecular Medicine
Florian M. Pauler - Austrian Acad Sci CeMM, Res Ctr Mol Med, Vienna, Austria
Doris Chen - CeMM Research Center for Molecular Medicine
Nicole Them - Austrian Acad Sci CeMM, Res Ctr Mol Med, Vienna, Austria
Fiorella Schischlik - Austrian Acad Sci CeMM, Res Ctr Mol Med, Vienna, Austria
Manuele Rebsamen - CeMM Research Center for Molecular Medicine
Michal Smida - Austrian Acad Sci CeMM, Res Ctr Mol Med, Vienna, Austria
Ferran Fece de la Cruz - Austrian Acad Sci CeMM, Res Ctr Mol Med, Vienna, Austria
Ana Lapao - Haplogen GmbH, Vienna, Austria
Melissa Liszt - Haplogen GmbH, Vienna, Austria
Benjamin Eizinger - Haplogen GmbH, Vienna, Austria
Philipp M. Guenzl - Austrian Acad Sci CeMM, Res Ctr Mol Med, Vienna, Austria
Vincent A. Blomen - Netherlands Canc Inst, Amsterdam, Netherlands
Tomasz Konopka - Austrian Acad Sci CeMM, Res Ctr Mol Med, Vienna, Austria
Bianca Gapp - Austrian Acad Sci CeMM, Res Ctr Mol Med, Vienna, Austria
Katja Parapatics - CeMM Research Center for Molecular Medicine
Barbara Maier - Austrian Acad Sci CeMM, Res Ctr Mol Med, Vienna, Austria
Johannes Stoeckl - Med Univ Vienna, Inst Immunol, Vienna, Austria
Wolfgang Fischl - Haplogen GmbH, Vienna, Austria
Sejla Salic - Haplogen GmbH, Vienna, Austria
M. Rita Taba Casari - Haplogen GmbH, Vienna, Austria
Sylvia Knapp - CeMM Research Center for Molecular Medicine
Keiryn L. Bennett - Austrian Acad Sci CeMM, Res Ctr Mol Med, Vienna, Austria
Christoph Bock - CeMM Research Center for Molecular Medicine
Jacques Colinge - Austrian Acad Sci CeMM, Res Ctr Mol Med, Vienna, Austria
Robert Kralovics - CeMM Research Center for Molecular Medicine
Gustav Ammerer - Univ Vienna, Max F Perutz Labs, Vienna, Austria
Georg Casari - Haplogen GmbH, Vienna, Austria
Thijn R. Brummelkamp - Austrian Acad Sci CeMM, Res Ctr Mol Med, Vienna, Austria
Giulio Superti-Furga - Austrian Acad Sci CeMM, Res Ctr Mol Med, Vienna, Austria
Sebastian M. B. Nijman - Austrian Acad Sci CeMM, Res Ctr Mol Med, Vienna, Austria
Détails de publication: Nature methods, Vol.10(10), pp.965-971
Éditeur: Springer Nature
Nombre de pages: 9
Note de subvention: Zentrum fur Innovation und Technologie Life Sciences ERC-2009-AdG-250179-i-FIVE / European Research Council grant; European Research Council (ERC) FemPower grant from Zentrum fur Innovation und Technologie (Die Technologieagentur der Stadt Wien) ALTF1346-2011 / European Molecular Biology Organization fellowship; European Molecular Biology Organization (EMBO)
Identifiants: 99140070109311
Unité académique: Institut de Recherche en Cancérologie de Montpellier - IRCM
Langue: English
Type de ressource: Journal article