A proteomic approach based on peptide affinity chromatography, 2-dimensional electrophoresis and mass spectrometry to identify multiprotein complexes interacting with membrane-bound receptors

Carine Bécamel; Nathalie Galéotti; Joël Poncet; Patrick Jouin; Aline Dumuis; Joël Bockaert; Philippe Marin

doi:10.1251/bpo39

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A proteomic approach based on peptide affinity chromatography, 2-dimensional electrophoresis and mass spectrometry to identify multiprotein complexes interacting with membrane-bound receptors

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A proteomic approach based on peptide affinity chromatography, 2-dimensional electrophoresis and mass spectrometry to identify multiprotein complexes interacting with membrane-bound receptors

Carine Bécamel, Nathalie Galéotti, Joël Poncet, Patrick Jouin, Aline Dumuis, Joël Bockaert et Philippe Marin

Biological procedures online, Vol.4(1), pp.94-104

09/12/2002

DOI: https://doi.org/10.1251/bpo39

PMID: 12734563

Résumé

There is accumulating evidence that membrane-bound receptors interact with many intracellular proteins. Multiprotein complexes associated with ionotropic receptors have been extensively characterized, but the identification of proteins interacting with G protein-coupled receptors (GPCRs) has so far only been achieved in a piecemeal fashion, focusing on one or two protein species. We describe a method based on peptide affinity chromatography, two-dimensional electrophoresis, mass spectrometry and immunoblotting to identify the components of multiprotein complexes interacting directly or indirectly with intracellular domains of GPCRs or, more generally, any other membrane-bound receptor. Using this global approach, we have characterized multiprotein complexes that bind to the carboxy-terminal tail of the 5-hydroxytryptamine type 2C receptor and are important for its subcellular localization in CNS cells (Bécamel et al., EMBO J., 21(10): 2332, 2002).

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Titre: A proteomic approach based on peptide affinity chromatography, 2-dimensional electrophoresis and mass spectrometry to identify multiprotein complexes interacting with membrane-bound receptors
Créateurs - sans rôle: Carine Bécamel - CNRS UPR 9023. 141, Rue de la Cardonille, 34094 Montpellier Cedex 5. France. Phone: +33 467 14 29 83 Fax: +33 467 14 29 10
Nathalie Galéotti - CNRS UPR 9023. 141, Rue de la Cardonille, 34094 Montpellier Cedex 5. France. Phone: +33 467 14 29 83 Fax: +33 467 14 29 10
Joël Poncet - CNRS UPR 9023. 141, Rue de la Cardonille, 34094 Montpellier Cedex 5. France. Phone: +33 467 14 29 83 Fax: +33 467 14 29 10
Patrick Jouin - CNRS UPR 9023. 141, Rue de la Cardonille, 34094 Montpellier Cedex 5. France. Phone: +33 467 14 29 83 Fax: +33 467 14 29 10
Aline Dumuis - CNRS UPR 9023. 141, Rue de la Cardonille, 34094 Montpellier Cedex 5. France. Phone: +33 467 14 29 83 Fax: +33 467 14 29 10
Joël Bockaert - CNRS UPR 9023. 141, Rue de la Cardonille, 34094 Montpellier Cedex 5. France. Phone: +33 467 14 29 83 Fax: +33 467 14 29 10
Philippe Marin - CNRS UPR 9023. 141, Rue de la Cardonille, 34094 Montpellier Cedex 5. France. Phone: +33 467 14 29 83 Fax: +33 467 14 29 10
Détails de publication: Biological procedures online, Vol.4(1), pp.94-104
Éditeur: Biological Procedures Online
Nombre de pages: 11
Identifiants: 99139427109311
Unité académique: Institut de Génomique Fonctionnelle - IGF
Langue: English
Type de ressource: Journal article