Abstract
The current biodiversity crisis calls for rapid and wide-ranging surveys to assess living organisms. However, some taxa are more elusive than others, making monitoring challenging. This is the case for soil invertebrates, but new molecular technologies such as eDNA metabarcoding could help to alleviate this problem. In this study, we evaluated the feasibility of using an eDNA approach to survey dung beetles, adapting existing monitoring methods for surveying dung fauna to enable eDNA collection in a non-destructive way. The main design idea is to capture species secretions and excretions from a serum-soaked nonwoven compress in a baited non-destructive trap. While the attractiveness of the device to dung beetles and the sampling protocol would benefit from further development, eDNA allowed the identification of more than 68% of trapped species and an identification of relative abundance match rate of 79%. The results of the study demonstrate the effectiveness of eDNA-based detection tools for the monitoring of dung beetles compared to standard surveying and identification techniques. Moreover, the adapted collecting device developed for the study could be used for similar surveys of other terrestrial invertebrates or even re-adapted. Ultimately, we hope this study encourages more non-invasive studies of insects by enabling others to utilize these emerging, non-destructive molecular techniques and therefore foster wide insect monitorings and conservation programs.