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A new technique for visualizing proanthocyanidins by light microscopy
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A new technique for visualizing proanthocyanidins by light microscopy

Jean Marc Brillouet et J. Escoute
Biotechnic and Histochemistry, Vol.87(3), pp.195 - 200
2012

Résumé

proanthocyanidins artifacts condensed tannins ethanol light microscopy localization VITIS-VINIFERA L. HISTOCHEMISTRY LOCALIZATION CELL-WALLS GRAPE BERRIES FIXATION FLAVAN-3-OLS CV CABERNET FRANC exogenous protein caffeine
We describe a new technique for visualizing proanthocyanidin-containing elements in plant tissues. Our innovation is the fixation of condensed tannins with an exogenous protein prior to alcohol dehydration. In this way, tannins do not undergo partial solubilization during the dehydration sequence and appear as sharply contoured globules of various diameters.

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