A new generation of cross-linkers for selective detection by MALDI MS

David Paramelle; Sonia Cantel; Christine Enjalbal; Muriel Amblard; Eric Forest; Michaël Heymann; Christophe Geourjon; Jean Martinez; Gilles Subra

doi:10.1002/pmic.200900562

Back

A new generation of cross-linkers for selective detection by MALDI MS

Journal article

Peer reviewed

A new generation of cross-linkers for selective detection by MALDI MS

David Paramelle, Sonia Cantel, Christine Enjalbal, Muriel Amblard, Eric Forest, Michaël Heymann, Christophe Geourjon, Jean Martinez and Gilles Subra

Proteomics, Vol.9, pp.5384-5388

2009

DOI: https://doi.org/10.1002/pmic.200900562

Abstract

Cross-linker

Matrix-assisted laser desorption

Protein structure prediction

Signal enhancement

Technology

UV-absorbing label

We designed a new cross-linker bearing a CHCA moiety. The use of the CHCA-tagged crosslinker JMV 3378 in conjunction with a neutral MALDI matrix a-cyano-4-hydroxycinnamic methyl ester enabled specific signal enhancement in MALDI-TOF MS of cross-link containing peptides. Discrimination between modified and non-modified peptides can be achieved by comparison of two spectra, one using CHCA and the other using the a-cyano-4- hydroxycinnamic methyl ester matrix. The methodology was validated using cytochrome c and apo-myoglobine as model proteins.

Files and links (1)

url

Find in HALView

Metrics

1 Record Views

Details

Title: A new generation of cross-linkers for selective detection by MALDI MS
Creators - without role: David Paramelle - Université de Montpellier, Institut des Biomolécules Max Mousseron - IBMM
Sonia Cantel - Université de Montpellier, Institut des Biomolécules Max Mousseron - IBMM
Christine Enjalbal - Université de Montpellier, Institut des Biomolécules Max Mousseron - IBMM
Muriel Amblard - Université de Montpellier, Institut des Biomolécules Max Mousseron - IBMM
Eric Forest - Institut de Biologie Structurale
Michaël Heymann - Institut de Biologie et de Chimie des Protéines
Christophe Geourjon - Institut de Biologie et de Chimie des Protéines
Jean Martinez - Université de Montpellier, Institut des Biomolécules Max Mousseron - IBMM
Gilles Subra - Université de Montpellier, Institut des Biomolécules Max Mousseron - IBMM
Publication Details: Proteomics, Vol.9, pp.5384-5388
Identifiers: 9944464309311
Academic Unit: Institut des Biomolécules Max Mousseron - IBMM
Language: English
Resource Type: Journal article
Local Fields: hal-00580764