Résumé
The glycoprotein 66 kD seen in Lane 4 (upper fraction from protein fraction of crude elicitor preparation subjected to ultrafiltration) is released by germlings of the canker stain agent of plane tree. This protein is the major eliciting compound for scopoletin and umbelliferone synthesis in cell-suspension cultures.
Treatment of cell-suspension cultures of
Plantanus × acerifolia with a crude elicitor preparation from
Ceratocystis fimbriata f. sp.
plantani germlings induced the synthesis of the hydroxycoumarin phytoalexins, scopoletin and umbelliferone, and their accumulation in the growth medium. Only the protein-containing fraction of the culture filtrate was involved in cell response. By ultrafiltration of this last fraction, a major eliciting glycoprotein able to induce 80% coumarin synthesis was isolated. The glycoprotein was substituted by N-glycan(s) containing terminally linked mannose as revealed by lectin immunoblotting. The molecular mass of the eliciting compound is 66 kD (SDS-PAGE) and the native conformation was necessary for elicitor recognition by plane tree cells and thereby phytoalexin synthesis. We discuss the possible involvement of the GP 66 elicitor from the canker stain agent of the plane tree in the activation of the phenolic metabolism of the plant.