Résumé
The potential regulatory signals contained in the
v-myb oncogene of avian myeloblastosis virus have been inserted upstream to the herpes simplex type 1 thymidine kinase gene in order to test their promoter activity. The isolation of TK
+ transformants after transfection of clone 1D (TK
−) mouse cells with the resulting recombinant DNAs indicated that expression of the TK gene was made possible by the
myb-derived sequences. Analysis of the TK specific RNA expressed in different TK
+ transformants revealed that the regulatory signals contained in
v-myb correspond to a weak functional promoter.