Résumé
T‐DNA integration in the nuclear plant genome may lead to rearrangements of the plant target site. Here we present evidence for a chromosomal inversion of 26 cM bordered by two T‐DNAs in direct orientation, which is linked to the
mgoun2
mutation. The integration sites of the T‐DNAs map at positions 80 and 106 of chromosome I and we show that each T‐DNA is bordered by plant sequences from positions 80 and 106, respectively. Although the T‐DNAs are physically distant, they are genetically closely linked. In addition, three markers located on the chromosome segment between the two T‐DNA integration sites show no recombination with the
mgo2
mutation. We show that the inversion cannot be a consequence of a recombination event between the two T‐DNAs, but that the integration of the T‐DNAs and the inversion were two temporally linked events. T‐DNA integration mechanisms that could have led to this inversion are discussed.